000088211 001__ 88211
000088211 005__ 20200716101444.0
000088211 0247_ $$2doi$$a10.1016/j.eimc.2019.01.015
000088211 0248_ $$2sideral$$a111264
000088211 037__ $$aART-2019-111264
000088211 041__ $$aspa
000088211 100__ $$aCeballos, S.
000088211 245__ $$aEstudio multicéntrico de cepas clínicas de SARM sensibles a antibióticos no-ß-lactámicos: líneas genéticas y producción de la leucocidina de Panton-Valentine
000088211 260__ $$c2019
000088211 5060_ $$aAccess copy available to the general public$$fUnrestricted
000088211 5203_ $$aIntroduction: Methicillin-resistant Staphylococcus aureus (MRSA) is considered a major cause of healthcare-associated (HA) and community-acquired (CA) infections. Considering non-ß-lactam susceptibility as a potential marker for mecC-MRSA and CA-MRSA, the aim of this study was to determine the frequency and the associated genetic lineages of non-beta-lactam-antibiotic susceptible MRSA (NBLS-MRSA) strains in a multicenter study in Spain.
Methods: A collection of 45 NBLS-MRSA strains recovered in the period from January to June 2016 from 12 Spanish hospitals was analyzed. Molecular typing through spa-type characterization, agr group and multi-locus-sequence typing was performed. Methicillin-resistant genes (mecA and mecC) as well as immune evasion cluster (scn-chp-sak-sea-sep, considering scn gene as the marker of IEC system) and Panton-Valentine leukocidin (PVL) genes were determined with PCR/sequencing.
Results: The NBLS-MRSA phenotype was uncommon in the 12 hospitals analyzed (NBLS-MRSA/MRSA frequency: 0.3%-7.7%). All strains contained the mecA gene (and none contained mecC). Twenty-two different spa-types were detected among NBLS-MRSA strains, with spa-t008/agr-I the most prevalent (27%). The main clonal complexes were (CC/%): CC8/42.2%, CC5/33.3% and CC30/4.4%, with ST8 and ST5 as the main sequence types. The PVL toxin was present in 38% of strains (with spa-types t008, t024, t019, t044, t068, t318 and t3060). The IEC genes were detected in 78% of strains: IEC type-B (n = 17), type-F (n = 16), type-A (n = 1) and type-E (n = 1); 10 MRSA isolates were scn-negative.
Conclusion: The NBLS-MRSA phenotype is uncommon in the analyzed hospitals; although no mecC-positive strains were detected, it could be a good marker for MRSA PVL-positive isolates (38%), frequently associated with CA-MRSA infections.
000088211 536__ $$9info:eu-repo/grantAgreement/ES/MINECO/SAF2016-76571-R
000088211 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc-nd$$uhttp://creativecommons.org/licenses/by-nc-nd/3.0/es/
000088211 590__ $$a1.654$$b2019
000088211 591__ $$aMICROBIOLOGY$$b112 / 134 = 0.836$$c2019$$dQ4$$eT3
000088211 591__ $$aINFECTIOUS DISEASES$$b79 / 93 = 0.849$$c2019$$dQ4$$eT3
000088211 592__ $$a0.317$$b2019
000088211 593__ $$aMicrobiology (medical)$$c2019$$dQ3
000088211 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/acceptedVersion
000088211 700__ $$aAspiroz, C.
000088211 700__ $$aRuiz-Ripa, L.
000088211 700__ $$aAzcona-Gutierrez, J.M.
000088211 700__ $$aLópez-Cerero, L.
000088211 700__ $$aLópez-Calleja, A.I.
000088211 700__ $$aÁlvarez, L.
000088211 700__ $$aGomáriz, M.
000088211 700__ $$aFernández, M.
000088211 700__ $$aTorres, C.
000088211 700__ $$aEzpeleta, C.
000088211 700__ $$aMartín, C.
000088211 700__ $$aArribas, J.
000088211 700__ $$aNavarro, C.
000088211 700__ $$aArias, A.
000088211 700__ $$aFortuño, B.
000088211 700__ $$aPereira, J.
000088211 700__ $$aMilagro, A.
000088211 700__ $$aTorres, L.
000088211 700__ $$aSoria-Blanco, L.M.
000088211 700__ $$aCanut, A.
000088211 700__ $$aCordón, M.L.
000088211 700__ $$aMegías, G.
000088211 700__ $$aCalvo, J.
000088211 700__ $$0(orcid)0000-0001-7294-245X$$aRezusta, A.$$uUniversidad de Zaragoza
000088211 7102_ $$11008$$2630$$aUniversidad de Zaragoza$$bDpto. Microb.Med.Pr.,Sal.Públ.$$cÁrea Microbiología
000088211 773__ $$g37, 8 (2019), 509-513$$pEnferm. infecc. microbiol. clín.$$tEnfermedades Infecciosas y Microbiologia Clinica$$x0213-005X
000088211 8564_ $$s249097$$uhttps://zaguan.unizar.es/record/88211/files/texto_completo.pdf$$yPostprint
000088211 8564_ $$s76034$$uhttps://zaguan.unizar.es/record/88211/files/texto_completo.jpg?subformat=icon$$xicon$$yPostprint
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000088211 951__ $$a2020-07-16-09:01:26
000088211 980__ $$aARTICLE