88372 20240322114949.0 doi 10.1016/j.foodcont.2019.03.035 sideral 111411 ART-2019-111411 eng Segura-Gil, I. Development of sandwich and competitive ELISA formats to determine ß-conglycinin: Evaluation of their performance to detect soy in processed food 2019 Access copy available to the general public Unrestricted Two ELISA formats (sandwich and indirect competitive) were developed to quantify ß-conglycinin, a major soy allergen. Their performance was evaluated using three model foods incurred with soy proteins. The sandwich format detects the addition of 0.005% and 0.05% soy proteins in pasteurized sausages and baked bread. However, the competitive format detects only 0.1 and 0.5%, respectively. ß-conglycinin was not detected in sterilized pâtè with any format. An industrial prototype of the sandwich ELISA was in-house validated, showing acceptable results of repeatability, reproducibility and robustness. Model foods spiked with ß-conglycinin after processing showed recoveries between 93.3 and 138.7%. However, in model foods incurred with soy proteins before processing the recovery decreased with the increase of the severity of heat treatment applied. The sandwich format could differentiate most of the retail foods with soy declared or not as ingredient. The ELISA format and processing conditions greatly influence the determination of ß-conglycinin in food. info:eu-repo/grantAgreement/ES/MINECO/RTC-2015-3818-2 info:eu-repo/semantics/openAccess by-nc-nd http://creativecommons.org/licenses/by-nc-nd/3.0/es/ 4.258 2019 FOOD SCIENCE & TECHNOLOGY 19 / 138 = 0.138 2019 Q1 T1 1.43 2019 Food Science 2019 Q1 Biotechnology 2019 Q1 info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion Blázquez-Soro, A. Galán-Malo, P. Mata, L. Tobajas, A.P. Universidad de Zaragoza (orcid)0000-0002-2646-5733 Sánchez, L. Universidad de Zaragoza (orcid)0000-0001-5964-823X Pérez, M.D. Universidad de Zaragoza (orcid)0000-0003-2555-8425 2008 780 Universidad de Zaragoza Dpto. Produc.Animal Cienc.Ali. Área Tecnología de Alimentos 103 (2019), 78-85 Food control Food Control 0956-7135 587449 http://zaguan.unizar.es/record/88372/files/texto_completo.pdf Postprint 210295 http://zaguan.unizar.es/record/88372/files/texto_completo.jpg?subformat=icon icon Postprint oai:zaguan.unizar.es:88372 articulos driver 2024-03-22-11:46:32 ARTICLE