000088391 001__ 88391 000088391 005__ 20200716101523.0 000088391 0247_ $$2doi$$a10.7589/2018-05-124 000088391 0248_ $$2sideral$$a111607 000088391 037__ $$aART-2019-111607 000088391 041__ $$aeng 000088391 100__ $$0(orcid)0000-0001-5028-947X$$aCalvete, C. 000088391 245__ $$aDetection of Rabbit Hemorrhagic Disease Virus GI.2/RHDV2/b in the Mediterranean Pine Vole ( Microtus duodecimcostatus) and White-Toothed Shrew ( Crocidura russula) 000088391 260__ $$c2019 000088391 5060_ $$aAccess copy available to the general public$$fUnrestricted 000088391 5203_ $$aThe European wild rabbit ( Oryctolagus cuniculus) is a key prey species on the Iberian Peninsula, and several predator species that are at risk of extinction are dependent on them as prey. A new rabbit hemorrhagic disease (RHD) virus genotype (GI.2/RHDV2/b) emerged in 2010 and posed a threat to wild rabbit populations. During a survey aimed at investigating RHD epidemiology in wild rabbits, GI.2/RHDV2/b was detected by duplex real-time PCR in carcasses of one Mediterranean pine vole ( Microtus duodecimcostatus) and two white-toothed shrews ( Crocidura russula). Laboratory New Zealand white rabbits that were challenged with inocula obtained from the liver of the small mammals died showing RHD lesions, confirming the infectiousness of the isolates. Phylogenetic analysis of the VP60 gene nucleotide sequences showed complete homology between the isolates from the two small mammal species and a high degree of similarity, but not complete homology, to GI.2/RHDV2/b sequences from wild rabbits. The GI.2/RHDV2/b genotype has not been reported in species outside the order Lagomorpha. 000088391 536__ $$9info:eu-repo/grantAgreement/ES/INIA/E-RTA2014-00009-00-00 000088391 540__ $$9info:eu-repo/semantics/openAccess$$aAll rights reserved$$uhttp://www.europeana.eu/rights/rr-f/ 000088391 590__ $$a1.187$$b2019 000088391 591__ $$aVETERINARY SCIENCES$$b66 / 142 = 0.465$$c2019$$dQ2$$eT2 000088391 592__ $$a0.672$$b2019 000088391 593__ $$aEcology, Evolution, Behavior and Systematics$$c2019$$dQ2 000088391 593__ $$aEcology$$c2019$$dQ2 000088391 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/acceptedVersion 000088391 700__ $$aMendoza, M. 000088391 700__ $$aSarto, M.P. 000088391 700__ $$0(orcid)0000-0002-5490-5177$$aJiménez de Bagüés, M.P. 000088391 700__ $$0(orcid)0000-0002-2053-9842$$aLuján, L.$$uUniversidad de Zaragoza 000088391 700__ $$0(orcid)0000-0001-5050-0167$$aMolín, J. 000088391 700__ $$aCalvo, A.J. 000088391 700__ $$aMonroy, F. 000088391 700__ $$0(orcid)0000-0001-9513-0219$$aCalvo, J.H. 000088391 7102_ $$11009$$2773$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Sanidad Animal 000088391 773__ $$g55, 2 (2019), 467-472$$pJ. wildl. dis.$$tJournal of wildlife diseases$$x0090-3558 000088391 8564_ $$s74984$$uhttps://zaguan.unizar.es/record/88391/files/texto_completo.pdf$$yPostprint 000088391 8564_ $$s12011$$uhttps://zaguan.unizar.es/record/88391/files/texto_completo.jpg?subformat=icon$$xicon$$yPostprint 000088391 909CO $$ooai:zaguan.unizar.es:88391$$particulos$$pdriver 000088391 951__ $$a2020-07-16-09:28:23 000088391 980__ $$aARTICLE