000088408 001__ 88408
000088408 005__ 20210902121636.0
000088408 0247_ $$2doi$$a10.1038/s41467-020-14794-z
000088408 0248_ $$2sideral$$a117056
000088408 037__ $$aART-2020-117056
000088408 041__ $$aeng
000088408 100__ $$0(orcid)0000-0003-3791-2997$$aGarcía-García, Ana$$uUniversidad de Zaragoza
000088408 245__ $$aStructural basis for substrate specificity and catalysis of a1,6-fucosyltransferase
000088408 260__ $$c2020
000088408 5060_ $$aAccess copy available to the general public$$fUnrestricted
000088408 5203_ $$aCore-fucosylation is an essential biological modification by which a fucose is transferred from GDP-ß-L-fucose to the innermost N-acetylglucosamine residue of N-linked glycans. A single human enzyme a1,6-fucosyltransferase (FUT8) is the only enzyme responsible for this modification via the addition of an a-1,6-linked fucose to N-glycans. To date, the details of substrate recognition and catalysis by FUT8 remain unknown. Here, we report the crystal structure of FUT8 complexed with GDP and a biantennary complex N-glycan (G0), which provides insight into both substrate recognition and catalysis. FUT8 follows an SN2 mechanism and deploys a series of loops and an a-helix which all contribute in forming the binding site. An exosite, formed by one of these loops and an SH3 domain, is responsible for the recognition of branched sugars, making contacts specifically to the a1,3 arm GlcNAc, a feature required for catalysis. This information serves as a framework for inhibitor design, and helps to assess its potential as a therapeutic target.
000088408 536__ $$9info:eu-repo/grantAgreement/ES/MINECO/RTC-2017-6126-1$$9info:eu-repo/grantAgreement/ES/MINECO/CTQ2017-90039-R$$9info:eu-repo/grantAgreement/ES/MINECO/CTQ2013-44367-C2-2-P$$9info:eu-repo/grantAgreement/ES/MINECO/BFU2016-75633-P$$9info:eu-repo/grantAgreement/ES/MICINN/RTI2018-099592-B-C21$$9info:eu-repo/grantAgreement/ES/MICINN-AEI/MDM-2017-0720$$9info:eu-repo/grantAgreement/EC/FP7/283570/EU/Transnational access and enhancement of integrated Biological Structure determination at synchrotron X-ray radiation facilities/BIOSTRUCT-X$$9info:eu-repo/grantAgreement/ES/DGA/LMP58-18$$9info:eu-repo/grantAgreement/ES/DGA/E34-R17
000088408 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000088408 590__ $$a14.919$$b2020
000088408 591__ $$aMULTIDISCIPLINARY SCIENCES$$b4 / 73 = 0.055$$c2020$$dQ1$$eT1
000088408 592__ $$a5.559$$b2020
000088408 593__ $$aBiochemistry, Genetics and Molecular Biology (miscellaneous)$$c2020$$dQ1
000088408 593__ $$aPhysics and Astronomy (miscellaneous)$$c2020$$dQ1
000088408 593__ $$aChemistry (miscellaneous)$$c2020$$dQ1
000088408 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000088408 700__ $$0(orcid)0000-0003-1892-5063$$aCeballos-Laita, Laura
000088408 700__ $$aSerna, Sonia
000088408 700__ $$aArtschwager, Raik
000088408 700__ $$aReichardt, Niels C.
000088408 700__ $$aCorzana, Francisco
000088408 700__ $$0(orcid)0000-0002-3122-9401$$aHurtado-Guerrero, Ramón$$uUniversidad de Zaragoza
000088408 7102_ $$11002$$2060$$aUniversidad de Zaragoza$$bDpto. Bioq.Biolog.Mol. Celular$$cÁrea Bioquímica y Biolog.Mole.
000088408 773__ $$g11 (2020), 973 1-9$$pNATURE COMMUNICATIONS$$tNature Communications$$x2041-1723
000088408 8564_ $$s2580725$$uhttps://zaguan.unizar.es/record/88408/files/texto_completo.pdf$$yVersión publicada
000088408 8564_ $$s58412$$uhttps://zaguan.unizar.es/record/88408/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000088408 909CO $$ooai:zaguan.unizar.es:88408$$particulos$$pdriver
000088408 951__ $$a2021-09-02-08:56:19
000088408 980__ $$aARTICLE