000088415 001__ 88415
000088415 005__ 20231006143257.0
000088415 0247_ $$2doi$$a10.3390/ijms21062233
000088415 0248_ $$2sideral$$a117111
000088415 037__ $$aART-2020-117111
000088415 041__ $$aeng
000088415 100__ $$0(orcid)0000-0002-5228-248X$$aSanz-Rubio, David
000088415 245__ $$aForkhead Box P3 Methylation and Expression in Men with Obstructive Sleep Apnea
000088415 260__ $$c2020
000088415 5060_ $$aAccess copy available to the general public$$fUnrestricted
000088415 5203_ $$aBACKGROUND: Epigenetic changes in obstructive sleep apnea (OSA) have been proposed as a mechanism for end-organ vulnerability. In children with OSA, Forkhead Box P3 (FOXP3) DNA methylation were associated with inflammatory biomarkers; however, the methylation pattern and its effect in the expression of this gene have not been tested in adults with OSA. METHODS: Plasma samples from subjects without comorbid conditions other than OSA were analyzed (the Epigenetics Status and Subclinical Atherosclerosis in Obstructive Sleep Apnea (EPIOSA) Study: NCT02131610). In 16 patients with severe OSA (Apnea-Hypopnea Index-AHI- > 30 events/h) and seven matched controls (AHI < 5), methylation of FOXP3 gen was evaluated by PCR of the promoter and by pyrosequencing of the intron 1 Treg-specific demethylated region (TSDR). In another 74 patients with OSA (AHI > 10) and 31 controls, we quantified FOXP3 protein expression by ELISA and gene expression by quantitative real-time PCR. C-reactive protein (CRP) and plasma Treg cells were also evaluated. RESULTS: Neither the levels of the promoter nor the TSDR demethylated region were different between controls and patients with OSA, whether they were grouped by normal or high CRP. FOXP3 protein and mRNA expression did not differ between groups. CONCLUSIONS: FOXP3 methylation or its expression is not altered in adults with OSA, whatever their inflammatory status.
000088415 536__ $$9info:eu-repo/grantAgreement/ES/DGA/A19-17R$$9info:eu-repo/grantAgreement/ES/ISCIII/PI12-01275
000088415 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000088415 590__ $$a5.923$$b2020
000088415 591__ $$aBIOCHEMISTRY & MOLECULAR BIOLOGY$$b67 / 296 = 0.226$$c2020$$dQ1$$eT1
000088415 591__ $$aCHEMISTRY, MULTIDISCIPLINARY$$b49 / 178 = 0.275$$c2020$$dQ2$$eT1
000088415 592__ $$a1.455$$b2020
000088415 593__ $$aCatalysis$$c2020$$dQ1
000088415 593__ $$aComputer Science Applications$$c2020$$dQ1
000088415 593__ $$aInorganic Chemistry$$c2020$$dQ1
000088415 593__ $$aSpectroscopy$$c2020$$dQ1
000088415 593__ $$aMolecular Biology$$c2020$$dQ1
000088415 593__ $$aOrganic Chemistry$$c2020$$dQ1
000088415 593__ $$aPhysical and Theoretical Chemistry$$c2020$$dQ1
000088415 593__ $$aMedicine (miscellaneous)$$c2020$$dQ1
000088415 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000088415 700__ $$0(orcid)0000-0001-6489-8399$$aSanz, Arianne$$uUniversidad de Zaragoza
000088415 700__ $$0(orcid)0000-0001-6256-5478$$aVarona, Luis$$uUniversidad de Zaragoza
000088415 700__ $$0(orcid)0000-0002-2746-3932$$aBolea, Rosa$$uUniversidad de Zaragoza
000088415 700__ $$aForner, Marta
000088415 700__ $$aGil, Aana V.
000088415 700__ $$aCubero, Pablo
000088415 700__ $$aMarin-Oto, Marta
000088415 700__ $$0(orcid)0000-0001-6016-4726$$aMartin-Burriel, Inmaculada$$uUniversidad de Zaragoza
000088415 700__ $$0(orcid)0000-0001-9096-2294$$aMarin, José M.$$uUniversidad de Zaragoza
000088415 7102_ $$11001$$2420$$aUniversidad de Zaragoza$$bDpto. Anatom.,Embri.Genét.Ani.$$cÁrea Genética
000088415 7102_ $$11009$$2773$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Sanidad Animal
000088415 7102_ $$11007$$2610$$aUniversidad de Zaragoza$$bDpto. Medicina, Psiqu. y Derm.$$cArea Medicina
000088415 773__ $$g21, 6 (2020), 2233 [12 pp.]$$pInt. j. mol. sci.$$tInternational Journal of Molecular Sciences$$x1661-6596
000088415 8564_ $$s1478127$$uhttps://zaguan.unizar.es/record/88415/files/texto_completo.pdf$$yVersión publicada
000088415 8564_ $$s492637$$uhttps://zaguan.unizar.es/record/88415/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000088415 909CO $$ooai:zaguan.unizar.es:88415$$particulos$$pdriver
000088415 951__ $$a2023-10-06-14:06:22
000088415 980__ $$aARTICLE