88415 20260112133139.0 doi 10.3390/ijms21062233 sideral 117111 ART-2020-117111 eng Sanz-Rubio, David (orcid)0000-0002-5228-248X Forkhead Box P3 Methylation and Expression in Men with Obstructive Sleep Apnea 2020 Access copy available to the general public Unrestricted BACKGROUND: Epigenetic changes in obstructive sleep apnea (OSA) have been proposed as a mechanism for end-organ vulnerability. In children with OSA, Forkhead Box P3 (FOXP3) DNA methylation were associated with inflammatory biomarkers; however, the methylation pattern and its effect in the expression of this gene have not been tested in adults with OSA. METHODS: Plasma samples from subjects without comorbid conditions other than OSA were analyzed (the Epigenetics Status and Subclinical Atherosclerosis in Obstructive Sleep Apnea (EPIOSA) Study: NCT02131610). In 16 patients with severe OSA (Apnea-Hypopnea Index-AHI- > 30 events/h) and seven matched controls (AHI < 5), methylation of FOXP3 gen was evaluated by PCR of the promoter and by pyrosequencing of the intron 1 Treg-specific demethylated region (TSDR). In another 74 patients with OSA (AHI > 10) and 31 controls, we quantified FOXP3 protein expression by ELISA and gene expression by quantitative real-time PCR. C-reactive protein (CRP) and plasma Treg cells were also evaluated. RESULTS: Neither the levels of the promoter nor the TSDR demethylated region were different between controls and patients with OSA, whether they were grouped by normal or high CRP. FOXP3 protein and mRNA expression did not differ between groups. CONCLUSIONS: FOXP3 methylation or its expression is not altered in adults with OSA, whatever their inflammatory status. info:eu-repo/grantAgreement/ES/DGA/A19-17R info:eu-repo/grantAgreement/ES/ISCIII/PI12-01275 info:eu-repo/semantics/openAccess by https://creativecommons.org/licenses/by/4.0/deed.es 5.923 2020 BIOCHEMISTRY & MOLECULAR BIOLOGY 67 / 296 = 0.226 2020 Q1 T1 CHEMISTRY, MULTIDISCIPLINARY 49 / 178 = 0.275 2020 Q2 T1 1.455 2020 Catalysis 2020 Q1 Computer Science Applications 2020 Q1 Inorganic Chemistry 2020 Q1 Spectroscopy 2020 Q1 Molecular Biology 2020 Q1 Organic Chemistry 2020 Q1 Physical and Theoretical Chemistry 2020 Q1 Medicine (miscellaneous) 2020 Q1 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Sanz, Arianne Universidad de Zaragoza (orcid)0000-0001-6489-8399 Varona, Luis Universidad de Zaragoza (orcid)0000-0001-6256-5478 Bolea, Rosa Universidad de Zaragoza (orcid)0000-0002-2746-3932 Forner, Marta Gil, Aana V. Cubero, Pablo Marin-Oto, Marta (orcid)0000-0003-0777-3850 Martin-Burriel, Inmaculada Universidad de Zaragoza (orcid)0000-0001-6016-4726 Marin, José M. Universidad de Zaragoza (orcid)0000-0001-9096-2294 1001 420 Universidad de Zaragoza Dpto. Anatom.,Embri.Genét.Ani. Área Genética 1009 773 Universidad de Zaragoza Dpto. Patología Animal Área Sanidad Animal 1007 610 Universidad de Zaragoza Dpto. Medicina, Psiqu. y Derm. Area Medicina 21, 6 (2020), 2233 [12 pp.] Int. j. mol. sci. International Journal of Molecular Sciences 1661-6596 1478127 http://zaguan.unizar.es/record/88415/files/texto_completo.pdf Versión publicada 492637 http://zaguan.unizar.es/record/88415/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:88415 articulos driver 2026-01-12-12:35:31 ARTICLE