88434 20200716101429.0 doi 10.1016/j.foodchem.2019.04.039 sideral 111409 ART-2019-111409 eng Galan-Malo, Patricia Development of a novel duplex lateral flow test for simultaneous detection of casein and ß-lactoglobulin in food 2019 Access copy available to the general public Unrestricted Milk by-products such as whey and caseinate are widely used as ingredients or processing aids in food industry. However, since they could cause allergic reactions they are included in Allergen Control Plans. ß-Lactoglobulin is the major whey protein and caseins are main proteins in milk. Selection of a unique target to analyze the presence of milk in foods could be insufficient when the source of milk proteins is unknown. A new test based on lateral flow immunocromatography that combines the simultaneous and independent detection of both proteins (ß-lactoglobulin and casein)in one rapid test was developed. The assay was validated according to AOAC guidelines being able to detect ß-lactoglobulin (0.5 ppm), casein (2 ppm), whey and powder milk (1–5 ppm). No cross-reactivity was found with a panel of 38 food commodities. The method is a rapid and suitable tool to identify milk proteins in processed food, ingredients, and rinsing water. info:eu-repo/grantAgreement/ES/MINECO/RTC-2015-3818-2 info:eu-repo/semantics/openAccess by-nc-nd http://creativecommons.org/licenses/by-nc-nd/3.0/es/ 6.306 2019 1.775 2019 CHEMISTRY, APPLIED 5 / 71 = 0.07 2019 Q1 T1 Analytical Chemistry 2019 Q1 NUTRITION & DIETETICS 10 / 89 = 0.112 2019 Q1 T1 Medicine (miscellaneous) 2019 Q1 FOOD SCIENCE & TECHNOLOGY 6 / 139 = 0.043 2019 Q1 T1 Food Science 2019 Q1 info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion Pellicer, Silvia (orcid)0000-0003-2555-8425 Pérez, María D. Universidad de Zaragoza (orcid)0000-0001-5964-823X Sánchez, Lourdes Universidad de Zaragoza Razquin, Pedro Mata, Luis 2008 780 Universidad de Zaragoza Dpto. Produc.Animal Cienc.Ali. Área Tecnología de Alimentos 293 (2019), 41-48 [30 p.] Food chem. Food Chemistry 0308-8146 399452 http://zaguan.unizar.es/record/88434/files/texto_completo.pdf Postprint 267735 http://zaguan.unizar.es/record/88434/files/texto_completo.jpg?subformat=icon icon Postprint oai:zaguan.unizar.es:88434 articulos driver 2020-07-16-08:47:39 ARTICLE