000088527 001__ 88527
000088527 005__ 20210902121625.0
000088527 0247_ $$2doi$$a10.3390/ijms21062093
000088527 0248_ $$2sideral$$a117233
000088527 037__ $$aART-2020-117233
000088527 041__ $$aeng
000088527 100__ $$0(orcid)0000-0001-6733-9373$$aMiguel-Jiménez, Sara$$uUniversidad de Zaragoza
000088527 245__ $$aDoes melatonin exert its effect on ram sperm capacitation through nitric oxide synthase regulation?
000088527 260__ $$c2020
000088527 5060_ $$aAccess copy available to the general public$$fUnrestricted
000088527 5203_ $$aNitric oxide (NO·), synthesized from L-arginine by nitric oxide synthase (NOS), is involved in sperm functionality. NOS isoforms have been detected in spermatozoa from different species, and an increment in NOS activity during capacitation has been reported. This work aims to determine the presence and localization of NOS isoforms in ram spermatozoa and analyse their possible changes during in vitro capacitation. Likewise, we investigated the effect of melatonin on the expression and localization of NOS and NO· levels in capacitated ram spermatozoa. Western blot analysis revealed protein bands associated with neuronal NOS (nNOS) and epithelial NOS (eNOS) but not with inducible NOS (iNOS). However, the three isoforms were detected by indirect immunofluorescence (IFI), and their immunotypes varied over in vitro capacitation with cAMP-elevating agents. NO· levels (evaluated by DAF-2-DA/PI staining) increased after in vitro capacitation, and the presence of L-arginine in the capacitating medium raised NO· production and enhanced the acrosome reaction. Incubation in capacitating conditions with a high-cAMP medium with melatonin modified the NOS distribution evaluated by IFI, but no differences in Western blotting were observed. Melatonin did not alter NO· levels in capacitating conditions, so we could infer that its role in ram sperm capacitation would not be mediated through NO· metabolism.
000088527 536__ $$9info:eu-repo/grantAgreement/ES/DGA/A07-17R$$9info:eu-repo/grantAgreement/ES/MINECO/AGL2017-83799-R
000088527 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000088527 590__ $$a5.923$$b2020
000088527 591__ $$aBIOCHEMISTRY & MOLECULAR BIOLOGY$$b67 / 297 = 0.226$$c2020$$dQ1$$eT1
000088527 591__ $$aCHEMISTRY, MULTIDISCIPLINARY$$b49 / 178 = 0.275$$c2020$$dQ2$$eT1
000088527 592__ $$a1.455$$b2020
000088527 593__ $$aCatalysis$$c2020$$dQ1
000088527 593__ $$aComputer Science Applications$$c2020$$dQ1
000088527 593__ $$aInorganic Chemistry$$c2020$$dQ1
000088527 593__ $$aSpectroscopy$$c2020$$dQ1
000088527 593__ $$aMolecular Biology$$c2020$$dQ1
000088527 593__ $$aOrganic Chemistry$$c2020$$dQ1
000088527 593__ $$aPhysical and Theoretical Chemistry$$c2020$$dQ1
000088527 593__ $$aMedicine (miscellaneous)$$c2020$$dQ1
000088527 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000088527 700__ $$0(orcid)0000-0003-3929-5064$$aCarvajal-Serna, Melissa$$uUniversidad de Zaragoza
000088527 700__ $$aCalvo, Silvia$$uUniversidad de Zaragoza
000088527 700__ $$0(orcid)0000-0003-1997-4262$$aCasao, Adriana$$uUniversidad de Zaragoza
000088527 700__ $$0(orcid)0000-0001-9842-6197$$aCebrián-Pérez, José Álvaro$$uUniversidad de Zaragoza
000088527 700__ $$0(orcid)0000-0003-1306-8517$$aMuiño-Blanco, Teresa$$uUniversidad de Zaragoza
000088527 700__ $$0(orcid)0000-0002-2312-6402$$aPérez-Pe, Rosaura$$uUniversidad de Zaragoza
000088527 7102_ $$11002$$2819$$aUniversidad de Zaragoza$$bDpto. Bioq.Biolog.Mol. Celular$$cÁrea Zoología
000088527 7102_ $$11011$$2630$$aUniversidad de Zaragoza$$bDpto. Microb.Ped.Radio.Sal.Pú.$$cÁrea Microbiología
000088527 7102_ $$11002$$2060$$aUniversidad de Zaragoza$$bDpto. Bioq.Biolog.Mol. Celular$$cÁrea Bioquímica y Biolog.Mole.
000088527 773__ $$g21, 6 (2020), 2093 [16 pp.]$$pInt. j. mol. sci.$$tInternational Journal of Molecular Sciences$$x1661-6596
000088527 8564_ $$s1439897$$uhttps://zaguan.unizar.es/record/88527/files/texto_completo.pdf$$yVersión publicada
000088527 8564_ $$s479030$$uhttps://zaguan.unizar.es/record/88527/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000088527 909CO $$ooai:zaguan.unizar.es:88527$$particulos$$pdriver
000088527 951__ $$a2021-09-02-08:49:48
000088527 980__ $$aARTICLE