000088583 001__ 88583
000088583 005__ 20210902121707.0
000088583 0247_ $$2doi$$a10.3390/antiox9020177
000088583 0248_ $$2sideral$$a117326
000088583 037__ $$aART-2020-117326
000088583 041__ $$aeng
000088583 100__ $$aCásedas, Guillermo
000088583 245__ $$aThe metabolite urolithin-a ameliorates oxidative stress in neuro-2a cells, becoming a potential neuroprotective agent
000088583 260__ $$c2020
000088583 5060_ $$aAccess copy available to the general public$$fUnrestricted
000088583 5203_ $$aUrolithin A is a metabolite generated from ellagic acid and ellagitannins by the intestinal microbiota after consumption of fruits such as pomegranates or strawberries. The objective of this study was to determine the cytoprotective capacity of this polyphenol in Neuro-2a cells subjected to oxidative stress, as well as its direct radical scavenging activity and properties as an inhibitor of oxidases. Cells treated with this compound and H2O2 showed a greater response to oxidative stress than cells only treated with H2O2, as mitochondrial activity (MTT assay), redox state (ROS formation, lipid peroxidation), and the activity of antioxidant enzymes (CAT: catalase, SOD: superoxide dismutase, GR: glutathione reductase, GPx: glutathione peroxidase) were significantly ameliorated; additionally, urolithin A enhanced the expression of cytoprotective peroxiredoxins 1 and 3. Urolithin A also acted as a direct radical scavenger, showing values of 13.2 µM Trolox Equivalents for Oxygen Radical Absorbance Capacity (ORAC) and 5.01 µM and 152.66 µM IC50 values for superoxide and 2, 2-diphenyss1-picrylhydrazyl (DPPH) radicals, respectively. Finally, inhibition of oxidizing enzymes, such as monoamine oxidase A and tyrosinase, was also detected in a dose-dependent manner. The cytoprotective effects of urolithin A could be attributed to the improvement of the cellular antioxidant battery, but also to its role as a direct radical scavenger and enzyme inhibitor of oxidases.
000088583 536__ $$9info:eu-repo/grantAgreement/ES/MICINN/RTI2018-094203-B-100
000088583 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000088583 590__ $$a6.312$$b2020
000088583 591__ $$aBIOCHEMISTRY & MOLECULAR BIOLOGY$$b60 / 297 = 0.202$$c2020$$dQ1$$eT1
000088583 591__ $$aFOOD SCIENCE & TECHNOLOGY$$b11 / 144 = 0.076$$c2020$$dQ1$$eT1
000088583 591__ $$aCHEMISTRY, MEDICINAL$$b6 / 63 = 0.095$$c2020$$dQ1$$eT1
000088583 592__ $$a1.066$$b2020
000088583 593__ $$aBiochemistry$$c2020$$dQ2
000088583 593__ $$aCell Biology$$c2020$$dQ2
000088583 593__ $$aPhysiology$$c2020$$dQ2
000088583 593__ $$aMolecular Biology$$c2020$$dQ2
000088583 593__ $$aClinical Biochemistry$$c2020$$dQ2
000088583 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000088583 700__ $$0(orcid)0000-0002-7972-7119$$aLes, Francisco
000088583 700__ $$aChoya-Foces, Carmen
000088583 700__ $$aHugo, Martín
000088583 700__ $$aLópez, Víctor
000088583 773__ $$g9, 2 (2020), 177 [16 pp.]$$pAntioxidants$$tAntioxidants$$x2076-3921
000088583 8564_ $$s842425$$uhttps://zaguan.unizar.es/record/88583/files/texto_completo.pdf$$yVersión publicada
000088583 8564_ $$s486595$$uhttps://zaguan.unizar.es/record/88583/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000088583 909CO $$ooai:zaguan.unizar.es:88583$$particulos$$pdriver
000088583 951__ $$a2021-09-02-09:18:01
000088583 980__ $$aARTICLE