000089610 001__ 89610
000089610 005__ 20210902121654.0
000089610 0247_ $$2doi$$a10.1038/s41598-020-64131-z
000089610 0248_ $$2sideral$$a117667
000089610 037__ $$aART-2020-117667
000089610 041__ $$aeng
000089610 100__ $$aValiente-Soriano, F.J.
000089610 245__ $$aTracing the retina to analyze the integrity and phagocytic capacity of the retinal pigment epithelium
000089610 260__ $$c2020
000089610 5060_ $$aAccess copy available to the general public$$fUnrestricted
000089610 5203_ $$aWe have developed a new technique to study the integrity, morphology and functionality of the retinal neurons and the retinal pigment epithelium (RPE). Young and old control albino (Sprague-Dawley) and pigmented (Piebald Virol Glaxo) rats, and dystrophic albino (P23H-1) and pigmented (Royal College of Surgeons) rats received a single intravitreal injection of 3% Fluorogold (FG) and their retinas were analyzed from 5 minutes to 30 days later. Retinas were imaged in vivo with SD-OCT and ex vivo in flat-mounts and in cross-sections. Fifteen minutes and 24 hours after intravitreal administration of FG retinal neurons and the RPE, but no glial cells, were labeled with FG-filled vesicles. The tracer reached the RPE 15 minutes after FG administration, and this labeling remained up to 30 days. Tracing for 15 minutes or 24 hours did not cause oxidative stress. Intraretinal tracing delineated the pathological retinal remodelling occurring in the dystrophic strains. The RPE of the P23H-1 strain was highly altered in aged animals, while the RPE of the RCS strain, which is unable to phagocytose, did not accumulate the tracer even at young ages when the retinal neural circuit is still preserved. In both dystrophic strains, the RPE cells were pleomorphic and polymegathic.
000089610 536__ $$9info:eu-repo/grantAgreement/ES/ISCIII-MINECO/PI16-00031$$9info:eu-repo/grantAgreement/ES/ISCIII-MINECO/PI16-00380$$9info:eu-repo/grantAgreement/ES/ISCIII-MINECO/PI19-00071$$9info:eu-repo/grantAgreement/ES/ISCIII-MINECO/PI19-00203$$9info:eu-repo/grantAgreement/ES/ISCIII-MINECO/RD16-0008$$9info:eu-repo/grantAgreement/ES/ISCIII-MINECO/RD16-0008-0016$$9info:eu-repo/grantAgreement/ES/ISCIII-MINECO/RD16-0008-0026$$9info:eu-repo/grantAgreement/ES/ISCIII-MINECO/SAF2015-67643-P
000089610 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000089610 590__ $$a4.379$$b2020
000089610 591__ $$aMULTIDISCIPLINARY SCIENCES$$b17 / 73 = 0.233$$c2020$$dQ1$$eT1
000089610 592__ $$a1.24$$b2020
000089610 593__ $$aMultidisciplinary$$c2020$$dQ1
000089610 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000089610 700__ $$aSalinas-Navarro, M.
000089610 700__ $$aDi Pierdomenico, J.
000089610 700__ $$aGarcía-Ayuso, D.
000089610 700__ $$aLucas-Ruiz, F.
000089610 700__ $$0(orcid)0000-0003-0349-9997$$aPinilla, I.$$uUniversidad de Zaragoza
000089610 700__ $$aCuenca, N.
000089610 700__ $$aVidal-Sanz, M.
000089610 700__ $$aVillegas-Pérez, M.P.
000089610 700__ $$aAgudo-Barriuso, M.
000089610 7102_ $$11013$$2646$$aUniversidad de Zaragoza$$bDpto. Cirugía$$cÁrea Oftalmología
000089610 773__ $$g10, 1 (2020), 7273 [18 pp]$$pSci. rep.$$tScientific Reports$$x2045-2322
000089610 8564_ $$s4199650$$uhttps://zaguan.unizar.es/record/89610/files/texto_completo.pdf$$yVersión publicada
000089610 8564_ $$s137254$$uhttps://zaguan.unizar.es/record/89610/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000089610 909CO $$ooai:zaguan.unizar.es:89610$$particulos$$pdriver
000089610 951__ $$a2021-09-02-09:09:14
000089610 980__ $$aARTICLE