000089647 001__ 89647
000089647 005__ 20210902121701.0
000089647 0247_ $$2doi$$a10.1016/j.postharvbio.2020.111208
000089647 0248_ $$2sideral$$a117718
000089647 037__ $$aART-2020-117718
000089647 041__ $$aeng
000089647 100__ $$0(orcid)0000-0003-1053-2556$$aCalvo, Héctor
000089647 245__ $$aAntifungal activity of the volatile organic compounds produced by Bacillus velezensis strains against postharvest fungal pathogens
000089647 260__ $$c2020
000089647 5060_ $$aAccess copy available to the general public$$fUnrestricted
000089647 5203_ $$aIt is known that Volatile Organic Compounds (VOCs), among several other mechanisms, are responsible for the antagonistic activity produced by microorganisms. In this work the volatilome of three biocontrol Bacillus velezensis strains (BUZ-14, I3 and I5) was tested in vitro and on fruit against Botrytis cinerea, Monilinia fructicola, M. laxa, Penicillium italicum, P. digitatum and P. expansum. In vitro, pathogens growth was significantly inhibited, in particular M. laxa, M. fructicola and P. italicum (66, 72 and 80 %, respectively) by BUZ-14 and B. cinerea (100 %) by I3 and I5, compared to the control. In vivo tests also showed significant inhibitions since volatile metabolites of I3 reduced grey mould in grapes by 50 % and those of BUZ-14 decreased brown rot severity in apricots, especially by M. fructicola, from 60 to 4 mm. VOCs were identified by solid phase microextraction (SPME) coupled with Gas Chromatography-Mass Spectrometry (GC–MS) and the antifungal activity of some of them was tested both in vitro and in vivo against the fungal pathogens. The main volatiles identified ranged from 12 to 15 compounds including 2-nonanone, 2-undecanone, 2-heptanone, 1-butanol, acetoin, benzaldehyde, butyl formate, diacetyl, nonane, or pyrazine, among others. Benzaldehyde and diacetyl obtained the lowest minimum inhibitory concentrations in vitro, ranging from 0.005 to 0.125 mL L-1 depending on the pathogen tested. Moreover, diacetyl was able to control grey mould caused by B. cinerea in table grapes with only 0.02 mL L-1 and to reduce blue rot in mandarins at the same dose up to 60 %. In this study, diacetyl and benzaldehyde have been identified as promising compounds to apply in active packaging during the postharvest commercialization of fruit. However, prior to the application, it is crucial to determine not only the active dose but also the phytotoxic, since some fruit species such as apples and apricots have proven to be highly sensitive.
000089647 536__ $$9info:eu-repo/grantAgreement/ES/DGA/LMP130-18
000089647 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc-nd$$uhttp://creativecommons.org/licenses/by-nc-nd/3.0/es/
000089647 590__ $$a5.537$$b2020
000089647 591__ $$aFOOD SCIENCE & TECHNOLOGY$$b20 / 144 = 0.139$$c2020$$dQ1$$eT1
000089647 591__ $$aAGRONOMY$$b6 / 91 = 0.066$$c2020$$dQ1$$eT1
000089647 591__ $$aHORTICULTURE$$b3 / 37 = 0.081$$c2020$$dQ1$$eT1
000089647 592__ $$a1.397$$b2020
000089647 593__ $$aAgronomy and Crop Science$$c2020$$dQ1
000089647 593__ $$aHorticulture$$c2020$$dQ1
000089647 593__ $$aFood Science$$c2020$$dQ1
000089647 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000089647 700__ $$0(orcid)0000-0001-8830-6554$$aMendiara, Isabel
000089647 700__ $$0(orcid)0000-0002-4843-2561$$aArias, Esther$$uUniversidad de Zaragoza
000089647 700__ $$0(orcid)0000-0001-9333-3055$$aGracia, Ana Pilar$$uUniversidad de Zaragoza
000089647 700__ $$0(orcid)0000-0001-6739-5375$$aBlanco, Domingo$$uUniversidad de Zaragoza
000089647 700__ $$0(orcid)0000-0001-6316-385X$$aVenturini, María Eugenia$$uUniversidad de Zaragoza
000089647 7102_ $$12008$$2640$$aUniversidad de Zaragoza$$bDpto. Produc.Animal Cienc.Ali.$$cÁrea Nutrición Bromatología
000089647 7102_ $$12008$$2780$$aUniversidad de Zaragoza$$bDpto. Produc.Animal Cienc.Ali.$$cÁrea Tecnología de Alimentos
000089647 773__ $$g166 (2020), 111208 [10 pp.]$$pPostharvest biol. technol.$$tPostharvest Biology and Technology$$x0925-5214
000089647 8564_ $$s1378588$$uhttps://zaguan.unizar.es/record/89647/files/texto_completo.pdf$$yVersión publicada
000089647 8564_ $$s71829$$uhttps://zaguan.unizar.es/record/89647/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000089647 909CO $$ooai:zaguan.unizar.es:89647$$particulos$$pdriver
000089647 951__ $$a2021-09-02-09:14:11
000089647 980__ $$aARTICLE