000089669 001__ 89669 000089669 005__ 20210902121706.0 000089669 0247_ $$2doi$$a10.3390/molecules25092023 000089669 0248_ $$2sideral$$a117773 000089669 037__ $$aART-2020-117773 000089669 041__ $$aeng 000089669 100__ $$aEstevez, María Belén 000089669 245__ $$aBiofilm eradication using biogenic silver nanoparticles 000089669 260__ $$c2020 000089669 5060_ $$aAccess copy available to the general public$$fUnrestricted 000089669 5203_ $$aMicroorganisms offer an alternative green and scalable technology for the synthesis of value added products. Fungi secrete high quantities of bioactive substances, which play dual-functional roles as both reducing and stabilizing agents in the synthesis of colloidal metal nanoparticles such as silver nanoparticles, which display potent antimicrobial properties that can be harnessed for a number of industrial applications. The aim of this work was the production of silver nanoparticles using the extracellular cell free extracts of Phanerochaete chrysosporium, and to evaluate their activity as antimicrobial and antibiofilm agents. The 45–nm diameter silver nanoparticles synthesized using this methodology possessed a high negative surface charge close to -30 mV and showed colloidal stability from pH 3–9 and under conditions of high ionic strength ([NaCl] = 10–500 mM). A combination of environmental SEM, TEM, and confocal Raman microscopy was used to study the nanoparticle-E. coli interactions to gain a first insight into their antimicrobial mechanisms. Raman data demonstrate a significant decrease in the fatty acid content of E. coli cells, which suggests a loss of the cell membrane integrity after exposure to the PchNPs, which is also commensurate with ESEM and TEM images. Additionally, these biogenic PchNPs displayed biofilm disruption activity for the eradication of E. coli and C. albicans biofilms. 000089669 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/ 000089669 590__ $$a4.411$$b2020 000089669 591__ $$aCHEMISTRY, MULTIDISCIPLINARY$$b63 / 178 = 0.354$$c2020$$dQ2$$eT2 000089669 591__ $$aBIOCHEMISTRY & MOLECULAR BIOLOGY$$b116 / 297 = 0.391$$c2020$$dQ2$$eT2 000089669 592__ $$a0.782$$b2020 000089669 593__ $$aAnalytical Chemistry$$c2020$$dQ1 000089669 593__ $$aChemistry (miscellaneous)$$c2020$$dQ1 000089669 593__ $$aDrug Discovery$$c2020$$dQ1 000089669 593__ $$aPhysical and Theoretical Chemistry$$c2020$$dQ1 000089669 593__ $$aMolecular Medicine$$c2020$$dQ1 000089669 593__ $$aOrganic Chemistry$$c2020$$dQ1 000089669 593__ $$aPharmaceutical Science$$c2020$$dQ1 000089669 593__ $$aMedicine (miscellaneous)$$c2020$$dQ1 000089669 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion 000089669 700__ $$aRaffaelli, Sofía 000089669 700__ $$0(orcid)0000-0003-4848-414X$$aMitchell, Scott G.$$uUniversidad de Zaragoza 000089669 700__ $$aFaccio, Ricardo 000089669 700__ $$aAlborés, Silvana 000089669 7102_ $$12013$$2765$$aUniversidad de Zaragoza$$bDpto. Química Orgánica$$cÁrea Química Orgánica 000089669 773__ $$g25, 9 (2020), 2023 [14 pp.]$$pMolecules$$tMolecules$$x1420-3049 000089669 8564_ $$s2106149$$uhttps://zaguan.unizar.es/record/89669/files/texto_completo.pdf$$yVersión publicada 000089669 8564_ $$s473285$$uhttps://zaguan.unizar.es/record/89669/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada 000089669 909CO $$ooai:zaguan.unizar.es:89669$$particulos$$pdriver 000089669 951__ $$a2021-09-02-09:17:05 000089669 980__ $$aARTICLE