89709 20230914083302.0 doi 10.3390/antibiotics9040175 sideral 117846 ART-2020-117846 eng Serrano, María Jesús (orcid)0000-0003-3850-9833 Is blood a good indicator for detecting antimicrobials in meat? Evidence for the development of in vivo surveillance methods 2020 Access copy available to the general public Unrestricted The introduction of antimicrobial residues in the food chain has a significant impact on human health. An innovative solution to avoid their presence in meat is the adaptation of current control methods for use with in vivo matrixes. Thus, the aim was to obtain paired blood and muscle samples from pigs treated with some of the main antimicrobials currently used in veterinary medicine (oxytetracycline, sulfamethoxypyridazine, enrofloxacin, amoxicillin), and to compare their rate of depletion in both matrixes. Antimicrobial concentrations in paired samples of blood and muscle were determined by liquid chromatography with tandem mass spectrometry (LC- MS/MS) or high performance liquid chromatography with fluorescence detection (HPLC-FLD). A comparison between values obtained in muscle and blood showed a similar distribution in both matrixes for oxytetracycline; for sulfamethoxypyridazine, a similar decrease rate but a concentration three times higher in blood compared to muscle was found; for enrofloxacin, we found significant differences in the rate of depletion, with similar antimicrobial concentrations in both matrixes with values close to the maximum residue limit (MRL) and higher amounts in muscle for values that lay considerably over the MRL. Conversely, amoxicillin depletion was so rapid that its appearance in carcasses does not seem to pose a risk. Therefore, blood would be a feasible matrix for the development of new in vivo tests. info:eu-repo/grantAgreement/EUR/INTERREG-V-A-POCTEFA/TESTACOS-EFA152/16 info:eu-repo/grantAgreement/EUR/INTERREG-V-A-POCTEFA-2014-2020 info:eu-repo/semantics/openAccess by http://creativecommons.org/licenses/by/3.0/es/ 4.639 2020 PHARMACOLOGY & PHARMACY 76 / 275 = 0.276 2020 Q2 T1 INFECTIOUS DISEASES 26 / 92 = 0.283 2020 Q2 T1 0.96 2020 Biochemistry 2020 Q1 Infectious Diseases 2020 Q1 Pharmacology, Toxicology and Pharmaceutics (miscellaneous) 2020 Q1 Microbiology (medical) 2020 Q1 Pharmacology (medical) 2020 Q1 Microbiology 2020 Q1 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Mitjana, Olga Universidad de Zaragoza (orcid)0000-0001-9907-2941 Bonastre, Cristina Universidad de Zaragoza (orcid)0000-0001-6178-9891 Laborda, Alicia Universidad de Zaragoza (orcid)0000-0003-0296-1617 Falceto, María Victoria Universidad de Zaragoza (orcid)0000-0001-6964-7011 García-Gonzalo, Diego Universidad de Zaragoza (orcid)0000-0002-7629-8101 Abilleira, Eunate Elorduy, Janire Bousquet-Melou, Alain Mata, Luis Condón, Santiago Universidad de Zaragoza (orcid)0000-0002-1902-0648 Pagán, Rafael Universidad de Zaragoza (orcid)0000-0002-0238-6328 1009 617 Universidad de Zaragoza Dpto. Patología Animal Área Medicina y Cirugía Animal 2008 780 Universidad de Zaragoza Dpto. Produc.Animal Cienc.Ali. Área Tecnología de Alimentos 9, 4 (2020), 175 [16 pp.] Antibiotics Antibiotics 2079-6382 625503 http://zaguan.unizar.es/record/89709/files/texto_completo.pdf Versión publicada 495508 http://zaguan.unizar.es/record/89709/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:89709 articulos driver 2023-09-13-10:54:05 ARTICLE