000089723 001__ 89723 000089723 005__ 20200716101547.0 000089723 0247_ $$2doi$$a10.3390/polym11101668 000089723 0248_ $$2sideral$$a114925 000089723 037__ $$aART-2019-114925 000089723 041__ $$aeng 000089723 100__ $$0(orcid)0000-0002-1505-498X$$aYus, Cristina$$uUniversidad de Zaragoza 000089723 245__ $$aTargeted release of probiotics from enteric microparticulated formulations 000089723 260__ $$c2019 000089723 5060_ $$aAccess copy available to the general public$$fUnrestricted 000089723 5203_ $$aThe development of advanced probiotic delivery systems, which preserve bacteria from degradation of the gastrointestinal tract and achieve a targeted release mediated by pH-independent swelling, is of great interest to improve the eficient delivery of probiotic bacteria to the target tissue. Gram-positive and Gram-negative bacteria models (Lactobacillus acidophilus (Moro) Hansen and Mocquot (ATCC®4356™) and Escherichia coli S17, respectively) have been successfully encapsulated for the first time in pH-independent microparticulate polymethacrylates (i.e., Eudraguard biotic) used for the targeted delivery of nutraceuticals to the colon. These bacteria have also been encapsulated within the mucoadhesive polymethacrylate Eudragit RS 100 widely used as targeted release formulation for active pharmaceutical ingredients. The enteric microparticles remained unaltered under simulated gastric conditions and released the contained viable microbial cargo under simulated intestinal conditions. Buoyancies of 90.2% and 57.3% for Eudragit and Eudraguard microparticles, respectively, and long-term stability (5 months) for the encapsulated microorganisms were found. Cytotoxicity of the microparticles formulated with both polymers was evaluated (0.5-20 mg/mL) on Caco-2 cells, showing high cytocompatibility. These results underline the suitability of the synthesized materials for the successful delivery of probiotic formulations to the target organ, highlighting for the first time the potential use of Eudraguard biotic as an effective enteric coating for the targeted delivery of probiotics. 000089723 536__ $$9info:eu-repo/grantAgreement/EC/FP7/614715/EU/A Photo-triggered On-demand Drug Delivery System for Chronic Pain/NANOHEDONISM 000089723 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/ 000089723 590__ $$a3.426$$b2019 000089723 591__ $$aPOLYMER SCIENCE$$b16 / 89 = 0.18$$c2019$$dQ1$$eT1 000089723 592__ $$a0.704$$b2019 000089723 593__ $$aPolymers and Plastics$$c2019$$dQ1 000089723 593__ $$aChemistry (miscellaneous)$$c2019$$dQ2 000089723 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion 000089723 700__ $$aGracia, Rubén 000089723 700__ $$0(orcid)0000-0002-6277-7996$$aLarrea, Ane 000089723 700__ $$0(orcid)0000-0002-6275-1104$$aAndreu, Vanesa 000089723 700__ $$0(orcid)0000-0002-2966-9088$$aIrusta, Silvia$$uUniversidad de Zaragoza 000089723 700__ $$0(orcid)0000-0002-6873-5244$$aSebastián, Víctor$$uUniversidad de Zaragoza 000089723 700__ $$0(orcid)0000-0003-2293-363X$$aMendoza, Gracia$$uUniversidad de Zaragoza 000089723 700__ $$0(orcid)0000-0003-3165-0156$$aArruebo, Manuel$$uUniversidad de Zaragoza 000089723 7102_ $$15005$$2555$$aUniversidad de Zaragoza$$bDpto. Ing.Quím.Tecnol.Med.Amb.$$cÁrea Ingeniería Química 000089723 7102_ $$15005$$2790$$aUniversidad de Zaragoza$$bDpto. Ing.Quím.Tecnol.Med.Amb.$$cÁrea Tecnologi. Medio Ambiente 000089723 773__ $$g11, 10 (2019), 1668 [14 pp.]$$pPolymers (Basel)$$tPolymers$$x2073-4360 000089723 8564_ $$s4852508$$uhttps://zaguan.unizar.es/record/89723/files/texto_completo.pdf$$yVersión publicada 000089723 8564_ $$s473880$$uhttps://zaguan.unizar.es/record/89723/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada 000089723 909CO $$ooai:zaguan.unizar.es:89723$$particulos$$pdriver 000089723 951__ $$a2020-07-16-09:43:47 000089723 980__ $$aARTICLE