89741 20210902121759.0 doi 10.1186/s13071-020-04114-1 sideral 118152 ART-2020-118152 eng Mignotte, A. The tree that hides the forest: Cryptic diversity and phylogenetic relationships in the Palaearctic vector Obsoletus/Scoticus Complex (Diptera: Ceratopogonidae) at the European level 2020 Access copy available to the general public Unrestricted Background: Culicoides obsoletus is an abundant and widely distributed Holarctic biting midge species, involved in the transmission of bluetongue virus (BTV) and Schmallenberg virus (SBV) to wild and domestic ruminants. Females of this vector species are often reported jointly with two morphologically very close species, C. scoticus and C. montanus, forming the Obsoletus/Scoticus Complex. Recently, cryptic diversity within C. obsoletus was reported in geographically distant sites. Clear delineation of species and characterization of genetic variability is mandatory to revise their taxonomic status and assess the vector role of each taxonomic entity. Our objectives were to characterize and map the cryptic diversity within the Obsoletus/Scoticus Complex. Methods: Portion of the cox1 mitochondrial gene of 3763 individuals belonging to the Obsoletus/Scoticus Complex was sequenced. Populations from 20 countries along a Palaearctic Mediterranean transect covering Scandinavia to Canary islands (North to South) and Canary islands to Turkey (West to East) were included. Genetic diversity based on cox1 barcoding was supported by 16S rDNA mitochondrial gene sequences and a gene coding for ribosomal 28S rDNA. Species delimitation using a multi-marker methodology was used to revise the current taxonomic scheme of the Obsoletus/Scoticus Complex. Results: Our analysis showed the existence of three phylogenetic clades (C. obsoletus clade O2, C. obsoletus clade dark and one not yet named and identified) within C. obsoletus. These analyses also revealed two intra-specific clades within C. scoticus and raised questions about the taxonomic status of C. montanus. Conclusions: To our knowledge, our study provides the first genetic characterization of the Obsoletus/Scoticus Complex on a large geographical scale and allows a revision of the current taxonomic classification for an important group of vector species of livestock viruses in the Palaearctic region.[Figure not available: See fulltext.] info:eu-repo/grantAgreement/EC/ECDC/OC-EFSA-AHAW-2013-02-FWC1 VectorNet info:eu-repo/grantAgreement/EC/H2020/727393/EU/Understanding pathogen, livestock, environment interactions involving bluetongue virus/PALE-Blu This project has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No H2020 727393-PALE-Blu info:eu-repo/semantics/openAccess by http://creativecommons.org/licenses/by/3.0/es/ 3.876 2020 TROPICAL MEDICINE 3 / 23 = 0.13 2020 Q1 T1 PARASITOLOGY 8 / 38 = 0.211 2020 Q1 T1 1.403 2020 Parasitology 2020 Q1 Infectious Diseases 2020 Q1 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Garros, C. Gardès, L. Balenghien, T. Duhayon, M. Rakotoarivony, I. Tabourin, L. Poujol, L. Mathieu, B. Ibañez-Justicia, A. Deniz, A. Cvetkovikj, A. Purse, B.V. Ramilo, D.W. Stougiou, D. Werner, D. Pudar, D. Petric, D. Veronesi, E. Jacobs, F. Kampen, H. Pereira Da Fonseca, I. Lucientes, J. Universidad de Zaragoza (orcid)0000-0003-0663-8411 Navarro, J. De La Puente, J.M. Stefanovska, J. Searle, K.R. Khallaayoune, K. Culverwell, C.L. Larska, M. Bourquia, M. Goffredo, M. Bisia, M. England, M. Robin, M. Quaglia, M. Miranda-Chueca, M.Á. Bødker, R. Estrada-Penã, R. Universidad de Zaragoza (orcid)0000-0001-7483-046X Carpenter, S. Tchakarova, S. Boutsini, S. Sviland, S. Schäfer, S.M. Ozolina&cedil , Z. Seglina&cedil , Z. Vatansever, Z. Huber, K. 1009 773 Universidad de Zaragoza Dpto. Patología Animal Área Sanidad Animal 13, 1 (2020), 265 [13 pp] Parasites & Vectors Parasites and Vectors 1756-3305 1800877 http://zaguan.unizar.es/record/89741/files/texto_completo.pdf Versión publicada 39628 http://zaguan.unizar.es/record/89741/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:89741 articulos driver 2021-09-02-09:53:54 ARTICLE