89754 20210902121712.0 doi 10.3390/ijms21082701 sideral 117854 ART-2020-117854 eng Fernández-alegre, Estela Melatonin non-linearly modulates bull spermatozoa motility and physiology in capacitating and non-capacitating conditions 2020 Access copy available to the general public Unrestricted Bull spermatozoa physiology may be modulated by melatonin. We washed ejaculated spermatozoa free of melatonin and incubated them (4 h, 38 °C) with 0-pM, 1-pM, 100-pM, 10-nM and 1-µM melatonin in TALP-HEPES (non-capacitating) and TALP-HEPES-heparin (capacitating). This range of concentrations encompassed the effects mediated by melatonin receptors (pM), intracellular targets (nM–µM) or antioxidant activity (µM). Treatment effects were assessed as motility changes by computer-assisted sperm analysis (CASA) of motility and physiological changes by flow cytometry. Melatonin effects were more evident in capacitating conditions, with 100 pM reducing motility and velocity (VCL) while increasing a “slow” subpopulation. All concentrations decreased apoptotic spermatozoa and stimulated mitochondrial activity in viable spermatozoa, with 100 pM–1 µM increasing acrosomal damage, 10 nM–1 µM increasing intracellular calcium and 1 pM reducing the response to a calcium-ionophore challenge. In non-capacitating media, 1 µM increased hyperactivation-related variables and decreased apoptotic spermatozoa; 100 pM–1 µM increased membrane disorders (related to capacitation); all concentrations decreased mitochondrial ROS production. Melatonin concentrations had a modal effect on bull spermatozoa, suggesting a capacitation-modulating role and protective effect at physiological concentrations (pM). Some effects may be of practical use, considering artificial reproductive techniques. info:eu-repo/grantAgreement/ES/MINECO-FEDER/AGL2013-43328-P info:eu-repo/semantics/openAccess by http://creativecommons.org/licenses/by/3.0/es/ 5.923 2020 BIOCHEMISTRY & MOLECULAR BIOLOGY 67 / 297 = 0.226 2020 Q1 T1 CHEMISTRY, MULTIDISCIPLINARY 49 / 178 = 0.275 2020 Q2 T1 1.455 2020 Catalysis 2020 Q1 Computer Science Applications 2020 Q1 Inorganic Chemistry 2020 Q1 Spectroscopy 2020 Q1 Molecular Biology 2020 Q1 Organic Chemistry 2020 Q1 Physical and Theoretical Chemistry 2020 Q1 Medicine (miscellaneous) 2020 Q1 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Álvarez-fernández, Indira Domínguez, Juan Carlos Casao, Adriana Universidad de Zaragoza (orcid)0000-0003-1997-4262 Martínez-pastor, Felipe 1002 819 Universidad de Zaragoza Dpto. Bioq.Biolog.Mol. Celular Área Zoología 21, 8 (2020), 2701 [20 pp.] Int. j. mol. sci. International Journal of Molecular Sciences 1661-6596 1724387 http://zaguan.unizar.es/record/89754/files/texto_completo.pdf Versión publicada 507970 http://zaguan.unizar.es/record/89754/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:89754 articulos driver 2021-09-02-09:21:48 ARTICLE