000089809 001__ 89809
000089809 005__ 20210902121818.0
000089809 0247_ $$2doi$$a10.1080/21505594.2020.1764173
000089809 0248_ $$2sideral$$a118275
000089809 037__ $$aART-2020-118275
000089809 041__ $$aeng
000089809 100__ $$0(orcid)0000-0002-8134-0693$$aArenas, J.$$uUniversidad de Zaragoza
000089809 245__ $$aIdentification of conditionally essential genes for Streptococcus suis infection in pigs
000089809 260__ $$c2020
000089809 5060_ $$aAccess copy available to the general public$$fUnrestricted
000089809 5203_ $$aStreptococcus suis is a Gram-positive bacterium and zoonotic pathogen that causes meningitis and sepsis in pigs and humans. The aim of this study was to identify genes required for S. suis infection. We created Tn-Seq libraries in a virulent S. suis strain 10, which was used to inoculate pigs in an intrathecal experimental infection. Comparative analysis of the relative abundance of mutants recovered from different sites of infection (blood, cerebrospinal fluid, and meninges of the brain) identified 361 conditionally essential genes, i.e. required for infection, which is about 18% of the genome. The conditionally essential genes were primarily involved in metabolic and transport processes, regulation, ribosomal structure and biogenesis, transcription, and cell wall membrane and envelope biogenesis, stress defenses, and immune evasion. Directed mutants were created in a set of 10 genes of different genetic ontologies and their role was determined in ex vivo models. Mutants showed different levels of sensitivity to survival in whole blood, serum, cerebrospinal fluid, thermic shock, and stress conditions, as compared to the wild type. Additionally, the role of three selected mutants was validated in co-infection experiments in which pigs were infected with both wild type and isogenic mutant strains. The genetic determinants of infection identified in this work contribute to novel insights in S. suis pathogenesis and could serve as targets for novel vaccines or antimicrobial drugs.
000089809 536__ $$9info:eu-repo/grantAgreement/EC/H2020/727966/EU/Program for Innovative Global Prevention of Streptococcus suis/PIGSs$$9This project has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No H2020 727966-PIGSs
000089809 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000089809 590__ $$a5.882$$b2020
000089809 591__ $$aINFECTIOUS DISEASES$$b12 / 92 = 0.13$$c2020$$dQ1$$eT1
000089809 591__ $$aMICROBIOLOGY$$b23 / 136 = 0.169$$c2020$$dQ1$$eT1
000089809 591__ $$aIMMUNOLOGY$$b44 / 162 = 0.272$$c2020$$dQ2$$eT1
000089809 592__ $$a1.927$$b2020
000089809 593__ $$aImmunology$$c2020$$dQ1
000089809 593__ $$aInfectious Diseases$$c2020$$dQ1
000089809 593__ $$aParasitology$$c2020$$dQ1
000089809 593__ $$aMicrobiology (medical)$$c2020$$dQ1
000089809 593__ $$aMicrobiology$$c2020$$dQ1
000089809 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000089809 700__ $$aZomer, A.
000089809 700__ $$aHarders-Westerveen, J.
000089809 700__ $$aBootsma, H.J.
000089809 700__ $$aDe Jonge, M.I.
000089809 700__ $$aStockhofe-Zurwieden, N.
000089809 700__ $$aSmith, H.E.
000089809 700__ $$aDe Greeff, A.
000089809 7102_ $$11009$$2773$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Sanidad Animal
000089809 773__ $$g11, 1 (2020), 446-464$$pVIRULENCE$$tVirulence$$x2150-5594
000089809 8564_ $$s4999030$$uhttps://zaguan.unizar.es/record/89809/files/texto_completo.pdf$$yVersión publicada
000089809 8564_ $$s227713$$uhttps://zaguan.unizar.es/record/89809/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000089809 909CO $$ooai:zaguan.unizar.es:89809$$particulos$$pdriver
000089809 951__ $$a2021-09-02-10:06:14
000089809 980__ $$aARTICLE