89811 20220426091135.0 doi 10.1111/1462-2920.15051 sideral 118278 ART-2020-118278 eng Wrobel, A. The inverse autotransporters of Yersinia ruckeri, YrInv and YrIlm, contribute to biofilm formation and virulence 2020 Access copy available to the general public Unrestricted Yersinia ruckeri causes enteric redmouth disease (ERM) that mainly affects salmonid fishes and leads to significant economic losses in the aquaculture industry. An increasing number of outbreaks and the lack of effective vaccines against some serotypes necessitates novel measures to control ERM. Importantly, Y. ruckeri survives in the environment for long periods, presumably by forming biofilms. How the pathogen forms biofilms and which molecular factors are involved in this process, remains unclear. Yersinia ruckeri produces two surface-exposed adhesins, belonging to the inverse autotransporters (IATs), called Y. ruckeri invasin (YrInv) and Y. ruckeri invasin-like molecule (YrIlm). Here, we investigated whether YrInv and YrIlm play a role in biofilm formation and virulence. Functional assays revealed that YrInv and YrIlm promote biofilm formation on different abiotic substrates. Confocal microscopy revealed that they are involved in microcolony interaction and formation, respectively. The effect of both IATs on biofilm formation correlated with the presence of different biopolymers in the biofilm matrix, including extracellular DNA, RNA and proteins. Moreover, YrInv and YrIlm contributed to virulence in the Galleria mellonella infection model. Taken together, we propose that both IATs are possible targets for the development of novel diagnostic and preventative strategies to control ERM. info:eu-repo/grantAgreement/EC/H2020/704903/EU/Bacterial Adhesion Control through Surface TArgeted Regulation/BAC-STAR This project has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No H2020 704903-BAC-STAR info:eu-repo/semantics/openAccess by http://creativecommons.org/licenses/by/3.0/es/ 5.491 2020 MICROBIOLOGY 30 / 136 = 0.221 2020 Q1 T1 1.954 2020 Microbiology 2020 Q1 Ecology, Evolution, Behavior and Systematics 2020 Q1 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Saragliadis, A. Pérez-Ortega, J. Sittman, C. Göttig, S. Liskiewicz, K. Spence, M.H. Schneider, K. Leo, J.C. Arenas, J. Universidad de Zaragoza (orcid)0000-0002-8134-0693 Linke, D. 1009 773 Universidad de Zaragoza Dpto. Patología Animal Área Sanidad Animal 22, 7 (2020), 2939-2955 Environ. microbiol. Environmental Microbiology 1462-2912 527069 http://zaguan.unizar.es/record/89811/files/texto_completo.pdf Versión publicada 48640 http://zaguan.unizar.es/record/89811/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:89811 articulos driver 2022-04-26-08:53:28 ARTICLE