000089841 001__ 89841
000089841 005__ 20211201113715.0
000089841 0247_ $$2doi$$a10.1016/j.cell.2017.05.029
000089841 0248_ $$2sideral$$a117555
000089841 037__ $$aART-2017-117555
000089841 041__ $$aeng
000089841 100__ $$aNozawa, R.S.
000089841 245__ $$aSAF-A Regulates Interphase Chromosome Structure through Oligomerization with Chromatin-Associated RNAs
000089841 260__ $$c2017
000089841 5060_ $$aAccess copy available to the general public$$fUnrestricted
000089841 5203_ $$aHigher eukaryotic chromosomes are organized into topologically constrained functional domains; however, the molecular mechanisms required to sustain these complex interphase chromatin structures are unknown. A stable matrix underpinning nuclear organization was hypothesized, but the idea was abandoned as more dynamic models of chromatin behavior became prevalent. Here, we report that scaffold attachment factor A (SAF-A), originally identified as a structural nuclear protein, interacts with chromatin-associated RNAs (caRNAs) via its RGG domain to regulate human interphase chromatin structures in a transcription-dependent manner. Mechanistically, this is dependent on SAF-A’s AAA+ ATPase domain, which mediates cycles of protein oligomerization with caRNAs, in response to ATP binding and hydrolysis. SAF-A oligomerization decompacts large-scale chromatin structure while SAF-A loss or monomerization promotes aberrant chromosome folding and accumulation of genome damage. Our results show that SAF-A and caRNAs form a dynamic, transcriptionally responsive chromatin mesh that organizes large-scale chromosome structures and protects the genome from instability.
000089841 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000089841 590__ $$a31.398$$b2017
000089841 591__ $$aCELL BIOLOGY$$b3 / 190 = 0.016$$c2017$$dQ1$$eT1
000089841 591__ $$aBIOCHEMISTRY & MOLECULAR BIOLOGY$$b2 / 292 = 0.007$$c2017$$dQ1$$eT1
000089841 592__ $$a25.137$$b2017
000089841 593__ $$aBiochemistry, Genetics and Molecular Biology (miscellaneous)$$c2017$$dQ1
000089841 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000089841 700__ $$aBoteva, L.
000089841 700__ $$aSoares, D.C.
000089841 700__ $$aNaughton, C.
000089841 700__ $$aDun, A.
000089841 700__ $$aBuckle, A.
000089841 700__ $$aRamsahoye, B.
000089841 700__ $$aBruton, P.C.
000089841 700__ $$aSaleeb, R.S.
000089841 700__ $$0(orcid)0000-0001-9962-2157$$aArnedo, M.
000089841 700__ $$aHill, B.
000089841 700__ $$aDuncan, R.R.
000089841 700__ $$aMaciver, S.K.
000089841 700__ $$aGilbert, N.
000089841 773__ $$g169, 7 (2017), 1214-1227$$pCell$$tCELL$$x0092-8674
000089841 8564_ $$s8580876$$uhttps://zaguan.unizar.es/record/89841/files/texto_completo.pdf$$yVersión publicada
000089841 8564_ $$s133089$$uhttps://zaguan.unizar.es/record/89841/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000089841 909CO $$ooai:zaguan.unizar.es:89841$$particulos$$pdriver
000089841 951__ $$a2021-12-01-11:35:16
000089841 980__ $$aARTICLE