89841 20211201113715.0 doi 10.1016/j.cell.2017.05.029 sideral 117555 ART-2017-117555 eng Nozawa, R.S. SAF-A Regulates Interphase Chromosome Structure through Oligomerization with Chromatin-Associated RNAs 2017 Access copy available to the general public Unrestricted Higher eukaryotic chromosomes are organized into topologically constrained functional domains; however, the molecular mechanisms required to sustain these complex interphase chromatin structures are unknown. A stable matrix underpinning nuclear organization was hypothesized, but the idea was abandoned as more dynamic models of chromatin behavior became prevalent. Here, we report that scaffold attachment factor A (SAF-A), originally identified as a structural nuclear protein, interacts with chromatin-associated RNAs (caRNAs) via its RGG domain to regulate human interphase chromatin structures in a transcription-dependent manner. Mechanistically, this is dependent on SAF-A’s AAA+ ATPase domain, which mediates cycles of protein oligomerization with caRNAs, in response to ATP binding and hydrolysis. SAF-A oligomerization decompacts large-scale chromatin structure while SAF-A loss or monomerization promotes aberrant chromosome folding and accumulation of genome damage. Our results show that SAF-A and caRNAs form a dynamic, transcriptionally responsive chromatin mesh that organizes large-scale chromosome structures and protects the genome from instability. info:eu-repo/semantics/openAccess by http://creativecommons.org/licenses/by/3.0/es/ 31.398 2017 CELL BIOLOGY 3 / 190 = 0.016 2017 Q1 T1 BIOCHEMISTRY & MOLECULAR BIOLOGY 2 / 292 = 0.007 2017 Q1 T1 25.137 2017 Biochemistry, Genetics and Molecular Biology (miscellaneous) 2017 Q1 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Boteva, L. Soares, D.C. Naughton, C. Dun, A. Buckle, A. Ramsahoye, B. Bruton, P.C. Saleeb, R.S. Arnedo, M. (orcid)0000-0001-9962-2157 Hill, B. Duncan, R.R. Maciver, S.K. Gilbert, N. 169, 7 (2017), 1214-1227 Cell CELL 0092-8674 8580876 http://zaguan.unizar.es/record/89841/files/texto_completo.pdf Versión publicada 133089 http://zaguan.unizar.es/record/89841/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:89841 articulos driver 2021-12-01-11:35:16 ARTICLE