89864 20240104111816.0 doi 10.3390/toxins12050330 sideral 118090 ART-2020-118090 eng Moreno-Martinez, L. Universidad de Zaragoza (orcid)0000-0001-5193-7782 Neuroprotective Fragment C of Tetanus Toxin Modulates IL-6 in an ALS Mouse Model 2020 Access copy available to the general public Unrestricted Neuroinflammation plays a significant role in amyotrophic lateral sclerosis (ALS) pathology, leading to the development of therapies targeting inflammation in recent years. Our group has studied the tetanus toxin C-terminal fragment (TTC) as a therapeutic molecule, showing neuroprotective properties in the SOD1G93A mouse model. However, it is unknown whether TTC could have some effect on inflammation. The objective of this study was to assess the effect of TTC on the regulation of inflammatory mediators to elucidate its potential role in modulating inflammation occurring in ALS. After TTC treatment in SOD1G93A mice, levels of eotaxin-1, interleukin (IL)-2, IL-6 and macrophage inflammatory protein (MIP)-1 alpha (a) and galectin-1 were analyzed by immunoassays in plasma samples, whilst protein expression of caspase-1, IL-1ß, IL-6 and NOD-, LRR- and pyrin domain-containing protein 3 (NLRP3) was measured in the spinal cord, extensor digitorum longus (EDL) muscle and soleus (SOL) muscle. The results showed reduced levels of IL-6 in spinal cord, EDL and SOL in treated SOD1G93A mice. In addition, TTC showed a different role in the modulation of NLRP3 and caspase-1 depending on the tissue analyzed. In conclusion, our results suggest that TTC could have a potential anti-inflammatory effect by reducing IL-6 levels in tissues drastically affected by the disease. However, further research is needed to study more in depth the anti-inflammatory effect of TTC in ALS. info:eu-repo/grantAgreement/ES/DGA/FSE info:eu-repo/grantAgreement/ES/FEDER/Una manera de hacer Europa info:eu-repo/grantAgreement/ES/FIS/PI17-00949 info:eu-repo/grantAgreement/ES/UZ-DGA/Grupos Consolidados info:eu-repo/semantics/openAccess by http://creativecommons.org/licenses/by/3.0/es/ 4.546 2020 TOXICOLOGY 21 / 93 = 0.226 2020 Q1 T1 FOOD SCIENCE & TECHNOLOGY 32 / 143 = 0.224 2020 Q1 T1 1.046 2020 Toxicology 2020 Q1 Health, Toxicology and Mutagenesis 2020 Q1 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion de la Torre, M. Universidad de Zaragoza (orcid)0000-0002-7955-7164 Muñoz, M.J. Universidad de Zaragoza (orcid)0000-0001-8301-6902 Zaragoza, P. Universidad de Zaragoza (orcid)0000-0001-5740-0185 Aguilera, J. Calvo, A.C. (orcid)0000-0002-7277-4318 Osta, R. Universidad de Zaragoza (orcid)0000-0001-5687-6704 1001 420 Universidad de Zaragoza Dpto. Anatom.,Embri.Genét.Ani. Área Genética 1012 315 Universidad de Zaragoza Dpto. Farmac.Fisiol.y Med.L.F. Área Farmacología 12, 5 (2020), 330 [10 pp] Toxins Toxins 2072-6651 630854 http://zaguan.unizar.es/record/89864/files/texto_completo.pdf Versión publicada 500829 http://zaguan.unizar.es/record/89864/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:89864 articulos driver 2024-01-04-11:05:08 ARTICLE