000094518 001__ 94518
000094518 005__ 20230914083253.0
000094518 0247_ $$2doi$$a10.3390/antibiotics9080440
000094518 0248_ $$2sideral$$a118540
000094518 037__ $$aART-2020-118540
000094518 041__ $$aeng
000094518 100__ $$aBuzón Durán, Laura
000094518 245__ $$aAntifungal activity against Fusarium culmorum of stevioside, Silybum marianum seed extracts, and their conjugate complexes
000094518 260__ $$c2020
000094518 5060_ $$aAccess copy available to the general public$$fUnrestricted
000094518 5203_ $$aFusarium head blight (FHB) is a disease that poses a major challenge in cereal production that has important food and feed safety implications due to trichothecene contamination. In this study, the effect of stevioside—a glycoside found in the leaves of candyleaf (Stevia rebaudiana Bertoni)—was evaluated in vitro against Fusarium culmorum (W.G. Smith) Sacc., alone and in combination (in a 1:1 molar ratio) with polyphenols obtained from milk thistle seeds (Silybum marianum (L.) Gaertn). Different concentrations, ranging from 32 to 512 µg·mL−1, were assayed, finding EC50 and EC90 inhibitory concentrations of 156 and 221 µg·mL−1, respectively, for the treatment based only on stevioside, and EC50 and EC90 values of 123 and 160 µg·mL−1, respectively, for the treatment based on the stevioside–polyphenol conjugate complexes. Colony formation inhibition results were consistent, reaching full inhibition at 256 µg·mL−1. Given that synergistic behavior was observed for this latter formulation (SF = 1.43, according to Wadley’s method), it was further assessed for grain protection at storage, mostly directed against mycotoxin contamination caused by the aforementioned phytopathogen, confirming that it could inhibit fungal growth and avoid trichothecene contamination. Moreover, seed tests showed that the treatment did not affect the percentage of germination, and it resulted in a lower incidence of root rot caused by the pathogen in Kamut and winter wheat seedlings. Hence, the application of these stevioside–S. marianum seed extract conjugate complexes may be put forward as a promising and environmentally friendly treatment for the protection of cereal crops and stored grain against FHB.
000094518 536__ $$9info:eu-repo/grantAgreement/ES/UZ/UZ2019-TEC-07
000094518 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000094518 590__ $$a4.639$$b2020
000094518 591__ $$aPHARMACOLOGY & PHARMACY$$b76 / 275 = 0.276$$c2020$$dQ2$$eT1
000094518 591__ $$aINFECTIOUS DISEASES$$b26 / 92 = 0.283$$c2020$$dQ2$$eT1
000094518 592__ $$a0.96$$b2020
000094518 593__ $$aBiochemistry$$c2020$$dQ1
000094518 593__ $$aInfectious Diseases$$c2020$$dQ1
000094518 593__ $$aPharmacology, Toxicology and Pharmaceutics (miscellaneous)$$c2020$$dQ1
000094518 593__ $$aMicrobiology (medical)$$c2020$$dQ1
000094518 593__ $$aPharmacology (medical)$$c2020$$dQ1
000094518 593__ $$aMicrobiology$$c2020$$dQ1
000094518 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000094518 700__ $$aMartín Gil, Jesús
000094518 700__ $$aRamos Sánchez, M. Carmen
000094518 700__ $$aPérez Lebeña, Eduardo
000094518 700__ $$aMarcos Robles, José Luis
000094518 700__ $$aFombellida Villafruela, Ángel
000094518 700__ $$0(orcid)0000-0003-2713-2786$$aMartín Ramos, Pablo$$uUniversidad de Zaragoza
000094518 7102_ $$15011$$2500$$aUniversidad de Zaragoza$$bDpto. CC.Agrar.y Medio Natural$$cArea Ingeniería Agroforestal
000094518 773__ $$g9, 8 (2020), 9080440 [14 pp.]$$pAntibiotics$$tAntibiotics$$x2079-6382
000094518 8564_ $$s2144915$$uhttps://zaguan.unizar.es/record/94518/files/texto_completo.pdf$$yVersión publicada
000094518 8564_ $$s505213$$uhttps://zaguan.unizar.es/record/94518/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000094518 909CO $$ooai:zaguan.unizar.es:94518$$particulos$$pdriver
000094518 951__ $$a2023-09-13-10:51:26
000094518 980__ $$aARTICLE