Circulating Exosomal Mir21 And Mir320 In Obstructive Sleep Apnea

Sanz-Rubio, D. ; Martin-Burrie, I. (Universidad de Zaragoza) ; Gil, V. ; Marin, J.M. (Universidad de Zaragoza)

Resumen: Rational. Epidemiological studies indicate that there may be an association between obstructive sleep apnea (OSA) and cardiovascular and metabolic diseases. Some pro-inflammatory miRs (miR-21, miR320) critical for the immune response or hypoxia are often overexpressed in cancers and atherosclerosis. Aim. To examine the expression of miR-21& miR320 in circulating exosomes from patients with OSA. Methods: From a Sleep Unit and in the frame of a long-term longitudinal cohort study we selected 65 non-smokers OSA patients (apnea-hypopnea index -AHI- 30 events/ti) and 26 age, gender and BMI-matched controls (AHI < 5). All participants were free of comorbidities other than OSA at baseline (GovTrials, NCT014575421). At recruitment and yearly, carotid ultrasound was performed and subclinical atherosclerosis (SA) was defined as by the presence of carotid plaques of by an intima-media thickness > 0.85 mm. Plasma-derived exosomes were isolated by precipitation using miRCURY, , , Exosome Isolation Kit. Exosomes were characterized by transmission electron microscopy, dynamic light scattering assay and Western Blot analysis using CD63 and HSP70. Exosome total RNA was obtained using miRCURY"* RNA isolation kit. miR-21 -5p and miR-320-3p were analysed by real time quantitative PCR (RT-qPCR) using miRCURY LNA~ technology...

Idioma: Inglés

Año: 2017

Publicado en: American Journal of Respiratory and Critical Care Medicine 195 (2017), A4523
ISSN: 1073-449X

Originalmente disponible en: Texto completo de la revista

Factor impacto JCR: 15.239 (2017)

Categ. JCR: RESPIRATORY SYSTEM rank: 2 / 59 = 0.034 (2017) - Q1 - T1
Categ. JCR: CRITICAL CARE MEDICINE rank: 2 / 33 = 0.061 (2017) - Q1 - T1

Factor impacto SCIMAGO: 5.942 - Pulmonary and Respiratory Medicine (Q1) - Critical Care and Intensive Care Medicine (Q1)

Financiación: info:eu-repo/grantAgreement/ES/ISCIII/FIS/PI12-02175
Financiación: info:eu-repo/grantAgreement/ES/ISCIII/FIS/PI15-1940

Tipo y forma: Comunicación congreso (Versión definitiva)

Área (Departamento): Área Genética (Dpto. Anatom.,Embri.Genét.Ani.)
Área (Departamento): Area Medicina (Dpto. Medicina, Psiqu. y Derm.)