94572 20231006143252.0 sideral 105667 ART-2017-105667 eng Sanz-Rubio, D. (orcid)0000-0002-5228-248X Circulating Exosomal Mir21 And Mir320 In Obstructive Sleep Apnea 2017 Access copy available to the general public Unrestricted Rational. Epidemiological studies indicate that there may be an association between obstructive sleep apnea (OSA) and cardiovascular and metabolic diseases. Some pro-inflammatory miRs (miR-21, miR320) critical for the immune response or hypoxia are often overexpressed in cancers and atherosclerosis. Aim. To examine the expression of miR-21& miR320 in circulating exosomes from patients with OSA. Methods: From a Sleep Unit and in the frame of a long-term longitudinal cohort study we selected 65 non-smokers OSA patients (apnea-hypopnea index -AHI- 30 events/ti) and 26 age, gender and BMI-matched controls (AHI < 5). All participants were free of comorbidities other than OSA at baseline (GovTrials, NCT014575421). At recruitment and yearly, carotid ultrasound was performed and subclinical atherosclerosis (SA) was defined as by the presence of carotid plaques of by an intima-media thickness > 0.85 mm. Plasma-derived exosomes were isolated by precipitation using miRCURY, , , Exosome Isolation Kit. Exosomes were characterized by transmission electron microscopy, dynamic light scattering assay and Western Blot analysis using CD63 and HSP70. Exosome total RNA was obtained using miRCURY"* RNA isolation kit. miR-21 -5p and miR-320-3p were analysed by real time quantitative PCR (RT-qPCR) using miRCURY LNA~ technology... info:eu-repo/grantAgreement/ES/ISCIII/FIS/PI12-02175 info:eu-repo/grantAgreement/ES/ISCIII/FIS/PI15-1940 info:eu-repo/semantics/openAccess All rights reserved http://www.europeana.eu/rights/rr-f/ 15.239 2017 RESPIRATORY SYSTEM 2 / 59 = 0.034 2017 Q1 T1 CRITICAL CARE MEDICINE 2 / 33 = 0.061 2017 Q1 T1 5.942 2017 Pulmonary and Respiratory Medicine 2017 Q1 Critical Care and Intensive Care Medicine 2017 Q1 info:eu-repo/semantics/conferenceObject info:eu-repo/semantics/publishedVersion Martin-Burrie, I. Universidad de Zaragoza (orcid)0000-0001-6016-4726 Gil, V. Marin, J.M. Universidad de Zaragoza (orcid)0000-0001-9096-2294 1001 420 Universidad de Zaragoza Dpto. Anatom.,Embri.Genét.Ani. Área Genética 1007 610 Universidad de Zaragoza Dpto. Medicina, Psiqu. y Derm. Area Medicina 195 (2017), A4523 Am. j. respir. crit. care med. American Journal of Respiratory and Critical Care Medicine 1073-449X 18174 http://zaguan.unizar.es/record/94572/files/texto_completo.pdf Versión publicada 426862 http://zaguan.unizar.es/record/94572/files/texto_completo.jpg?subformat=icon icon Versión publicada https://www.atsjournals.org/doi/abs/10.1164/ajrccm-conference.2017.195.1_MeetingAbstracts.A4523 Texto completo de la revista oai:zaguan.unizar.es:94572 articulos driver 2023-10-06-14:06:01 ARTICLE