000094573 001__ 94573 000094573 005__ 20230421130007.0 000094573 0247_ $$2doi$$a10.1007/s10787-019-00626-0 000094573 0248_ $$2sideral$$a112623 000094573 037__ $$aART-2020-112623 000094573 041__ $$aeng 000094573 100__ $$0(orcid)0000-0002-2231-7565$$aValero, Marta Sofía$$uUniversidad de Zaragoza 000094573 245__ $$aJasonia glutinosa (L.) DC., a traditional herbal medicine, reduces inflammation, oxidative stress and protects the intestinal barrier in a murine model of colitis 000094573 260__ $$c2020 000094573 5060_ $$aAccess copy available to the general public$$fUnrestricted 000094573 5203_ $$aJasonia glutinosa (L.) DC., known as rock tea (RT), is traditionally used in Spain as a digestive due to its beneficial properties in bowel disorders. The pharmacological nature of these properties has not been established yet. The aim of this work was to evaluate the therapeutic utility of RT in experimental colitis and to identify chemical constituents with anti-inflammatory and/or anti-oxidative properties. RT extract was prepared with ethanol in a Soxhlet apparatus and analysed by HPLC–DAD. Superoxide radical scavenging properties, xanthine oxidase and lipoxygenase (5-LOX) inhibitory activity, and capability to lower nitric oxide (NO) and tumor necrosis factor a (TNF-a) levels were measured in cell-free and cell-based assays. In the 2.5%-dextran-sodium sulphate (DSS) injury-repair model of ulcerative colitis (UC), mice were daily treated with sulfasalazine (SSZ, as reference drug, 100 mg/kg bw), RT (5, 25 and 50 mg/kg bw, p.o.), or vehicle over 20 days. Colitis was scored daily. Colon samples were examined macroscopically and histopathologically. Protein levels of myeloperoxidase (MPO), interleukins 6, and 10 (IL-6, IL-10), inducible NO synthase (iNOS), and cyclooxygenase-2 (COX-2) were studied as markers of oxidative stress and inflammatory activity. The integrity of the apical epithelial layer was assessed by immunofluorescence staining of zonula ocludens-1 (ZO-1). Finally, intestinal contractility was also evaluated by isometric myography. Fifteen phenolic compounds and three pigments were identified and quantified, of which caffeoylquinic acids, and the flavonoid, quercetin-3-O-galactoside, were the most abundant. RT extract significantly scavenged superoxide radicals, inhibited 5-LOX activity, and lowered NO and TNF-a levels. DSS-treated mice receiving RT scored clinically lower than controls during the first 3 days of DSS treatment and during the recovery period. SSZ was less effective than RT. Anatomical and histological examination of colon samples revealed that RT significantly prevented colon shortening, increased colon thickness, and lowered the macroscopic damage score. RT also significantly prevented the increase of MPO activity, IL-6 levels, iNOS and COX-2 expression, the loss of ZO-1 apical expression, and normalized contractility disturbances. In conclusion, daily administration of RT showed therapeutic properties in the DSS-model of UC. The benefits of RT can likely be attributed to its anti-inflammatory and antioxidant phenolic and flavonoid constituents. 000094573 536__ $$9info:eu-repo/grantAgreement/ES/DGA/B61$$9info:eu-repo/grantAgreement/ES/DGA/E02$$9info:eu-repo/grantAgreement/ES/UZ/JIUZ-2015-BIO-02$$9info:eu-repo/grantAgreement/ES/UZ/JIUZ-2018-BIO-09 000094573 540__ $$9info:eu-repo/semantics/openAccess$$aAll rights reserved$$uhttp://www.europeana.eu/rights/rr-f/ 000094573 590__ $$a4.473$$b2020 000094573 591__ $$aTOXICOLOGY$$b23 / 93 = 0.247$$c2020$$dQ1$$eT1 000094573 591__ $$aIMMUNOLOGY$$b71 / 162 = 0.438$$c2020$$dQ2$$eT2 000094573 592__ $$a0.944$$b2020 000094573 593__ $$aImmunology$$c2020$$dQ2 000094573 593__ $$aPharmacology (medical)$$c2020$$dQ2 000094573 593__ $$aPharmacology$$c2020$$dQ2 000094573 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/acceptedVersion 000094573 700__ $$aGonzález, Mateo 000094573 700__ $$aGimenez, Mariano Ramón 000094573 700__ $$aAndrade, Paula B. 000094573 700__ $$0(orcid)0000-0002-0182-201X$$aMoreo, Eduardo$$uUniversidad de Zaragoza 000094573 700__ $$aLes, Francisco 000094573 700__ $$aFernandes, Fátima 000094573 700__ $$aGómez-Rincón, Carlota 000094573 700__ $$aBerzosa, César 000094573 700__ $$aGarcía de Jalón, José Antonio 000094573 700__ $$0(orcid)0000-0002-2114-0577$$aArruebo, Mª. Pilar$$uUniversidad de Zaragoza 000094573 700__ $$0(orcid)0000-0002-7412-2073$$aPlaza, Miguel Ángel$$uUniversidad de Zaragoza 000094573 700__ $$aKöhler, Ralf 000094573 700__ $$aLópez, Víctor 000094573 700__ $$aValentão, Patricia 000094573 700__ $$0(orcid)0000-0001-8584-3979$$aCastro, Marta$$uUniversidad de Zaragoza 000094573 7102_ $$11012$$2410$$aUniversidad de Zaragoza$$bDpto. Farmac.Fisiol.y Med.L.F.$$cÁrea Fisiología 000094573 7102_ $$11011$$2630$$aUniversidad de Zaragoza$$bDpto. Microb.Ped.Radio.Sal.Pú.$$cÁrea Microbiología 000094573 773__ $$g28 (2020), 1717–1734$$pInflammopharmacology.$$tINFLAMMOPHARMACOLOGY$$x0925-4692 000094573 8564_ $$s329378$$uhttps://zaguan.unizar.es/record/94573/files/texto_completo.pdf$$yPostprint 000094573 8564_ $$s216479$$uhttps://zaguan.unizar.es/record/94573/files/texto_completo.jpg?subformat=icon$$xicon$$yPostprint 000094573 909CO $$ooai:zaguan.unizar.es:94573$$particulos$$pdriver 000094573 951__ $$a2023-04-21-12:40:49 000094573 980__ $$aARTICLE