000095032 001__ 95032
000095032 005__ 20210223082304.0
000095032 0247_ $$2doi$$a10.1111/lam.12815
000095032 0248_ $$2sideral$$a104208
000095032 037__ $$aART-2018-104208
000095032 041__ $$aeng
000095032 100__ $$0(orcid)0000-0003-3752-4660$$aPagán, E.$$uUniversidad de Zaragoza
000095032 245__ $$aAntimicrobial activity of suspensions and nanoemulsions of citral in combination with heat or pulsed electric fields
000095032 260__ $$c2018
000095032 5060_ $$aAccess copy available to the general public$$fUnrestricted
000095032 5203_ $$aThe application of essential oils in form of nanoemulsions has been proposed as a method to improve their solubility in aqueous solutions, and hence their antimicrobial activity. The objective of this study was to evaluate the antimicrobial activity of citral, applied directly or in combined treatments with heat or pulsed electric fields (PEF), as a function of the inoculation procedure assayed: (i) a simple, vigorous shaking method by vortex agitation (suspension of citral; s-citral) or (ii) the previous preparation of nanoemulsions by the emulsion phase inversion (EPI) method (nanoemulsion of citral; n-citral). n-Citral was more effective in either inhibiting or inactivating Escherichia coli O157:H7 Sakai than s-citral. However, when combined with heat, a greater synergistic effect was observed with s-citral rather than with n-citral, either in lab media (pH 7·0 and 4·0) or apple juice. For instance, while almost 5 log10 cell cycles were inactivated in apple juice after 15 min at 53°C in the presence of 0·1 µl ml-1 of s-citral, the use of n-citral required 30 min. The use of nanoemulsions did not modify the slight synergism observed when citral and mild PEF were combined (150 µs, 30 kV cm-1). Significance and Impact of the Study: The exploration of different delivery systems of antimicrobial compounds such as citral in aqueous food products aids in the establishment of successful combined treatments for food preservation. While at room temperature, citral in form of a nanoemulsion shows a higher antimicrobial activity; its combination with heat would imply a partial loss of the outstanding synergistic lethal effect achieved when added in suspension form. Therefore, the most suitable procedure to magnify the synergism between heat and citral when processing juices would merely require an intense homogenization step prior to the combined treatment.
000095032 536__ $$9info:eu-repo/grantAgreement/ES/MINECO/AGL2015-69565-P
000095032 540__ $$9info:eu-repo/semantics/openAccess$$aAll rights reserved$$uhttp://www.europeana.eu/rights/rr-f/
000095032 590__ $$a1.805$$b2018
000095032 591__ $$aBIOTECHNOLOGY & APPLIED MICROBIOLOGY$$b111 / 162 = 0.685$$c2018$$dQ3$$eT3
000095032 591__ $$aMICROBIOLOGY$$b103 / 133 = 0.774$$c2018$$dQ4$$eT3
000095032 592__ $$a0.573$$b2018
000095032 593__ $$aApplied Microbiology and Biotechnology$$c2018$$dQ2
000095032 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/acceptedVersion
000095032 700__ $$0(orcid)0000-0001-5053-8309$$aBerdejo, D.$$uUniversidad de Zaragoza
000095032 700__ $$aEspina, L.
000095032 700__ $$0(orcid)0000-0002-7629-8101$$aGarcía-Gonzalo, D.$$uUniversidad de Zaragoza
000095032 700__ $$0(orcid)0000-0002-0238-6328$$aPagán, R.$$uUniversidad de Zaragoza
000095032 7102_ $$12008$$2780$$aUniversidad de Zaragoza$$bDpto. Produc.Animal Cienc.Ali.$$cÁrea Tecnología de Alimentos
000095032 773__ $$g66, 1 (2018), 63-70$$pLett. appl. microbiol.$$tLETTERS IN APPLIED MICROBIOLOGY$$x0266-8254
000095032 8564_ $$s215631$$uhttps://zaguan.unizar.es/record/95032/files/texto_completo.pdf$$yPostprint
000095032 8564_ $$s144088$$uhttps://zaguan.unizar.es/record/95032/files/texto_completo.jpg?subformat=icon$$xicon$$yPostprint
000095032 909CO $$ooai:zaguan.unizar.es:95032$$particulos$$pdriver
000095032 951__ $$a2021-02-23-08:20:37
000095032 980__ $$aARTICLE