000095035 001__ 95035
000095035 005__ 20200904094319.0
000095035 0247_ $$2doi$$a10.1093/jac/dkz346
000095035 0248_ $$2sideral$$a115376
000095035 037__ $$aART-2019-115376
000095035 041__ $$aeng
000095035 100__ $$aDel Barrio-Tofiño, Ester
000095035 245__ $$aAssociation between Pseudomonas aeruginosa O-antigen serotypes, resistance profiles and high-risk clones: results from a Spanish nationwide survey
000095035 260__ $$c2019
000095035 5060_ $$aAccess copy available to the general public$$fUnrestricted
000095035 5203_ $$aObjectives: To evaluate the correlation of O-antigen serotypes with resistance profiles and high-risk clones in a Spanish nationwide survey.
Methods: Up to 30 consecutive healthcare-associated Pseudomonas aeruginosa isolates were collected during October 2017 from each of 51 hospitals (covering all Spanish regions) with a total of 1445 isolates studied. MICs of 13 antipseudomonal agents and MDR/XDR profiles had been previously determined, as well as whole-genome sequences of 185 representative XDR isolates. O-antigen serotypes (O1–O16) were determined by agglutination using serotype-specific antisera (BioRad). The Pseudomonas aeruginosa serotyper (PAst) program was used for in silico serotyping.
Results: The most frequent serotypes were O6 (17.8%), O1 (15.4%) and O11 (13.3%). In contrast, the most frequent serotype among XDR isolates (17.3%) was O4 (34.1%), distantly followed by O11 (15.9%). Within serotypes, XDR phenotypes were more frequent for O12 (60.0%) and O4 (57.3%). The most frequent clone among the XDR isolates was ST175 (40.9%), followed by CC235 (10.7%), ST308 (5.2%) and CC111 (3.6%). Up to 81.6%of XDR ST175 isolates typed O4, whereas 18.4% were non-typeable. O4 genotype was detected in all sequenced (n=55) ST175 isolates. On the other hand, CC235 and ST308 were associated with O11, whereas CC111 was linked to serotype O12.
Conclusions: O4 serotype is linked to the MDR/XDR profile of widespread ST175 (typically only susceptible to colistin, amikacin and the novel combinations ceftolozane/tazobactam and ceftazidime/avibactam) and therefore, after local validation, its detection in the microbiology laboratory might be useful for guiding semi-empirical antipseudomonal therapies and infection control measures in Spanish hospitals.
000095035 536__ $$9info:eu-repo/grantAgreement/ES/ISCIII/PI15-00088$$9info:eu-repo/grantAgreement/ES/ISCIII/PI18-00076$$9info:eu-repo/grantAgreement/ES/MINECO-FEDER/ISCIII-REIPI-RD16-0016
000095035 540__ $$9info:eu-repo/semantics/openAccess$$aAll rights reserved$$uhttp://www.europeana.eu/rights/rr-f/
000095035 590__ $$a5.439$$b2019
000095035 591__ $$aINFECTIOUS DISEASES$$b10 / 93 = 0.108$$c2019$$dQ1$$eT1
000095035 591__ $$aPHARMACOLOGY & PHARMACY$$b22 / 270 = 0.081$$c2019$$dQ1$$eT1
000095035 591__ $$aMICROBIOLOGY$$b24 / 134 = 0.179$$c2019$$dQ1$$eT1
000095035 592__ $$a2.23$$b2019
000095035 593__ $$aInfectious Diseases$$c2019$$dQ1
000095035 593__ $$aPharmacology (medical)$$c2019$$dQ1
000095035 593__ $$aPharmacology$$c2019$$dQ1
000095035 593__ $$aMicrobiology (medical)$$c2019$$dQ1
000095035 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/acceptedVersion
000095035 700__ $$aSánchez-Diener, Irina
000095035 700__ $$aZamorano, Laura
000095035 700__ $$aCortes-Lara, Sara
000095035 700__ $$aLópez-Causapé, Carla
000095035 700__ $$aCabot, Gabriel
000095035 700__ $$aBou, Germán
000095035 700__ $$aMartínez-Martínez, Luis
000095035 700__ $$aOliver, Antonio
000095035 700__ $$aon behalf of the GEMARA-SEIMC/REIPI Pseudomonas study group
000095035 700__ $$0(orcid)0000-0002-2519-701X$$aCastillo-García, Francisco Javier$$uUniversidad de Zaragoza
000095035 700__ $$0(orcid)0000-0002-9742-1463$$aSeral, Cristina$$uUniversidad de Zaragoza
000095035 7102_ $$11008$$2630$$aUniversidad de Zaragoza$$bDpto. Microb.Med.Pr.,Sal.Públ.$$cÁrea Microbiología
000095035 773__ $$g74, 11 (2019), 3217-3220$$pJ. antimicrob. chemother.$$tThe Journal of antimicrobial chemotherapy$$x1460-2091
000095035 8564_ $$s141090$$uhttps://zaguan.unizar.es/record/95035/files/texto_completo.pdf$$yPostprint
000095035 8564_ $$s50331$$uhttps://zaguan.unizar.es/record/95035/files/texto_completo.jpg?subformat=icon$$xicon$$yPostprint
000095035 909CO $$ooai:zaguan.unizar.es:95035$$particulos$$pdriver
000095035 951__ $$a2020-09-04-08:30:34
000095035 980__ $$aARTICLE