000095044 001__ 95044
000095044 005__ 20200904094319.0
000095044 0247_ $$2doi$$a10.1080/21505594.2019.1599669
000095044 0248_ $$2sideral$$a118553
000095044 037__ $$aART-2019-118553
000095044 041__ $$aeng
000095044 100__ $$0(orcid)0000-0002-8134-0693$$aArenas, Jesús$$uUniversidad de Zaragoza
000095044 245__ $$aIn vivo transcriptomes of Streptococcus suis reveal genes required for niche-specific adaptation and pathogenesis
000095044 260__ $$c2019
000095044 5060_ $$aAccess copy available to the general public$$fUnrestricted
000095044 5203_ $$aStreptococcus suis is a Gram-positive bacterium and a zoonotic pathogen residing in the nasopharynx or the gastrointestinal tract of pigs with a potential of causing life-threatening invasive disease. It is endemic in the porcine production industry worldwide, and it is also an emerging human pathogen. After invasion, the pathogen adapts to cause bacteremia and disseminates to different organs including the brain. To gain insights in this process, we infected piglets with a highly virulent strain of S. suis, and bacterial transcriptomes were obtained from blood and different organs (brain, joints, and heart) when animals had severe clinical symptoms of infection. Microarrays were used to determine the genome-wide transcriptional profile at different infection sites and during growth in standard growth medium in vitro. We observed differential expression of around 30% of the Open Reading Frames (ORFs) and infection-site specific patterns of gene expression. Genes with major changes in expression were involved in transcriptional regulation, metabolism, nutrient acquisition, stress defenses, and virulence, amongst others, and results were confirmed for a subset of selected genes using RT-qPCR. Mutants were generated in two selected genes, and the encoded proteins, i.e., NADH oxidase and MetQ, were shown to be important virulence factors in coinfection experiments and in vitro assays. The knowledge derived from this study regarding S. suis gene expression in vivo and identification of virulence factors is important for the development of novel diagnostic and therapeutic strategies to control S. suis disease.
000095044 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000095044 590__ $$a5.542$$b2019
000095044 591__ $$aIMMUNOLOGY$$b33 / 158 = 0.209$$c2019$$dQ1$$eT1
000095044 591__ $$aMICROBIOLOGY$$b22 / 134 = 0.164$$c2019$$dQ1$$eT1
000095044 591__ $$aINFECTIOUS DISEASES$$b9 / 93 = 0.097$$c2019$$dQ1$$eT1
000095044 592__ $$a1.757$$b2019
000095044 593__ $$aImmunology$$c2019$$dQ1
000095044 593__ $$aInfectious Diseases$$c2019$$dQ1
000095044 593__ $$aParasitology$$c2019$$dQ1
000095044 593__ $$aMicrobiology (medical)$$c2019$$dQ1
000095044 593__ $$aMicrobiology$$c2019$$dQ1
000095044 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000095044 700__ $$aBossers-de Vries, R.
000095044 700__ $$aHarders-Westerveen, José
000095044 700__ $$aBuys, Herma
000095044 700__ $$aRuuls-van Stalle, Lisette
000095044 700__ $$aStockhofe-Zurwieden, Norbert
000095044 700__ $$aZaccaria, Edoardo
000095044 700__ $$aTommassen, Jan
000095044 700__ $$aWells, Jerry M.
000095044 700__ $$aSmith, Hilde E.
000095044 700__ $$ade Greef, Astrid
000095044 7102_ $$11009$$2773$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Sanidad Animal
000095044 773__ $$g10, 1 (2019), 334 - 351$$pVIRULENCE$$tVirulence$$x2150-5594
000095044 8564_ $$s5119283$$uhttps://zaguan.unizar.es/record/95044/files/texto_completo.pdf$$yVersión publicada
000095044 8564_ $$s257154$$uhttps://zaguan.unizar.es/record/95044/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000095044 909CO $$ooai:zaguan.unizar.es:95044$$particulos$$pdriver
000095044 951__ $$a2020-09-04-08:31:20
000095044 980__ $$aARTICLE