000095348 001__ 95348 000095348 005__ 20210902121834.0 000095348 0247_ $$2doi$$a10.3389/fpls.2020.00819 000095348 0248_ $$2sideral$$a119182 000095348 037__ $$aART-2020-119182 000095348 041__ $$aeng 000095348 100__ $$aMoreno-Aguilar, M.F.$$uUniversidad de Zaragoza 000095348 245__ $$aMuseomics Unveil the Phylogeny and Biogeography of the Neglected Juan Fernandez Archipelago Megalachne and Podophorus Endemic Grasses and Their Connection With Relict Pampean-Ventanian Fescues 000095348 260__ $$c2020 000095348 5060_ $$aAccess copy available to the general public$$fUnrestricted 000095348 5203_ $$aOceanic islands constitute natural laboratories to study plant speciation and biogeographic patterns of island endemics. Juan Fernandez is a southern Pacific archipelago consisting of three small oceanic islands located 600–700 km west of the Chilean coastline. Exposed to current cold seasonal oceanic climate, these 5.8–1 Ma old islands harbor a remarkable endemic flora. All known Fernandezian endemic grass species belong to two genera, Megalachne and Podophorus, of uncertain taxonomic adscription. Classical and modern classifications have placed them either in Bromeae (Bromus), Duthieinae, Aveneae/Poeae, or Loliinae (fine-leaved Festuca); however, none of them have clarified their evolutionary relationships with respect to their closest Festuca relatives. Megalachne includes four species, which are endemic to Masatierra (Robinson Crusoe island) (M. berteroniana and M. robinsoniana) and to Masafuera (Alejandro Selkirk island) (M. masafuerana and M. dantonii). The monotypic Podophorus bromoides is a rare endemic species to Masatierra which is only known from its type locality and is currently considered extinct. We have used museomic approaches to uncover the challenging evolutionary history of these endemic grasses and to infer the divergence and dispersal patterns from their ancestors. Genome skimming data were produced from herbarium samples of M. berteroniana and M. masafuerana, and the 164 years old type specimen of P. bromoides, as well as for a collection of 33 species representing the main broad- and fine-leaved Loliinae lineages. Paired-end reads were successfully mapped to plastomes and nuclear ribosomal cistrons of reference Festuca species and used to reconstruct phylogenetic trees. Filtered ITS and trnTLF sequences from these genomes were further combined with our large Loliinae data sets for accurate biogeographic reconstruction. Nuclear and plastome data recovered a strongly supported fine-leaved Fernandezian clade where Podophorus was resolved as sister to Megalachne. Bayesian divergence dating and dispersal-extinction-cladogenesis range evolution analyses estimated the split of the Fernandezian clade from its ancestral southern American Pampas-Ventanian Loliinae lineage in the Miocene-Pliocene transition, following a long distance dispersal from the continent to the uplifted volcanic palaeo-island of Santa Clara-Masatierra. Consecutive Pliocene-Pleistocene splits and a Masatierra-to-Masafuera dispersal paved the way for in situ speciation of Podophorus and Megalachne taxa. 000095348 536__ $$9info:eu-repo/grantAgreement/ES/DGA/A01 000095348 540__ $$9info:eu-repo/semantics/openAccess$$aAll rights reserved$$uhttp://www.europeana.eu/rights/rr-f/ 000095348 590__ $$a5.753$$b2020 000095348 591__ $$aPLANT SCIENCES$$b17 / 235 = 0.072$$c2020$$dQ1$$eT1 000095348 592__ $$a1.752$$b2020 000095348 593__ $$aPlant Science$$c2020$$dQ1 000095348 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion 000095348 700__ $$aArnelas, I. 000095348 700__ $$aSánchez-Rodríguez, A. 000095348 700__ $$aViruel, J. 000095348 700__ $$0(orcid)0000-0001-7793-5259$$aCatalán, P.$$uUniversidad de Zaragoza 000095348 7102_ $$15011$$2063$$aUniversidad de Zaragoza$$bDpto. CC.Agrar.y Medio Natural$$cÁrea Botánica 000095348 773__ $$g11 (2020), 819 [18 pp.]$$pFront. plant sci.$$tFrontiers in Plant Science$$x1664-462X 000095348 8564_ $$s1248216$$uhttps://zaguan.unizar.es/record/95348/files/texto_completo.pdf$$yVersión publicada 000095348 8564_ $$s511713$$uhttps://zaguan.unizar.es/record/95348/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada 000095348 909CO $$ooai:zaguan.unizar.es:95348$$particulos$$pdriver 000095348 951__ $$a2021-09-02-10:18:42 000095348 980__ $$aARTICLE