000095351 001__ 95351
000095351 005__ 20240105103347.0
000095351 0247_ $$2doi$$a10.3389/fmicb.2020.01339
000095351 0248_ $$2sideral$$a119189
000095351 037__ $$aART-2020-119189
000095351 041__ $$aeng
000095351 100__ $$0(orcid)0000-0001-7897-9173$$aAguilo, N.$$uUniversidad de Zaragoza
000095351 245__ $$aRespiratory immunization with a whole cell inactivated vaccine induces functional mucosal immunoglobulins against tuberculosis in mice and non-human primates
000095351 260__ $$c2020
000095351 5060_ $$aAccess copy available to the general public$$fUnrestricted
000095351 5203_ $$aVaccination through the natural route of infection represents an attractive immunization strategy in vaccinology. In the case of tuberculosis, vaccine delivery by the respiratory route has regained interest in recent years, showing efficacy in different animal models. In this context, respiratory vaccination triggers lung immunological mechanisms which are omitted when vaccines are administered by parenteral route. However, contribution of mucosal antibodies to vaccine- induced protection has been poorly studied. In the present study, we evaluated in mice and non-human primates (NHP) a novel whole cell inactivated vaccine (MTBVAC HK), by mucosal administration. MTBVAC HK given by intranasal route to BCG-primed mice substantially improved the protective efficacy conferred by subcutaneous BCG only. Interestingly, this improved protection was absent in mice lacking polymeric Ig receptor (pIgR), suggesting a crucial role of mucosal secretory immunoglobulins in protective immunity. Our study in NHP confirmed the ability of MTBVAC HK to trigger mucosal immunoglobulins. Importantly, in vitro assays demonstrated the functionality of these immunoglobulins to induce M. tuberculosis opsonization in the presence of human macrophages. Altogether, our results suggest that mucosal immunoglobulins can be induced by vaccination to improve protection against tuberculosis and therefore, they represent a promising target for next generation tuberculosis vaccines.
000095351 536__ $$9info:eu-repo/grantAgreement/EC/H2020/643381/EU/TBVAC2020; Advancing novel and promising TB vaccine candidates from discovery to preclinical and early clinical development/TBVAC2020$$9This project has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No H2020 643381-TBVAC2020$$9info:eu-repo/grantAgreement/ES/MICINN/RTI2018-097625-B-100$$9info:eu-repo/grantAgreement/ES/MINECO/IPT2012-0327-090000
000095351 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000095351 590__ $$a5.64$$b2020
000095351 591__ $$aMICROBIOLOGY$$b28 / 135 = 0.207$$c2020$$dQ1$$eT1
000095351 592__ $$a1.701$$b2020
000095351 593__ $$aMicrobiology (medical)$$c2020$$dQ1
000095351 593__ $$aMicrobiology$$c2020$$dQ1
000095351 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000095351 700__ $$0(orcid)0000-0001-7866-2803$$aUranga, S.$$uUniversidad de Zaragoza
000095351 700__ $$aMata, E.$$uUniversidad de Zaragoza
000095351 700__ $$0(orcid)0000-0002-3086-3013$$aTarancon, R.$$uUniversidad de Zaragoza
000095351 700__ $$0(orcid)0000-0002-9713-2127$$aGómez, A.B.$$uUniversidad de Zaragoza
000095351 700__ $$0(orcid)0000-0001-8644-120X$$aMarinova, D.$$uUniversidad de Zaragoza
000095351 700__ $$0(orcid)0000-0003-1027-7583$$aOtal, I.$$uUniversidad de Zaragoza
000095351 700__ $$0(orcid)0000-0002-2787-9671$$aMonzón, M.$$uUniversidad de Zaragoza
000095351 700__ $$0(orcid)0000-0002-7173-7216$$aBadiola, J.$$uUniversidad de Zaragoza
000095351 700__ $$aMontenegro, D.
000095351 700__ $$aPuentes, E.
000095351 700__ $$aRodríguez, E.
000095351 700__ $$aVervenne, R.A.W.
000095351 700__ $$aSombroek, C.C.
000095351 700__ $$aVerreck, F.A.W.
000095351 700__ $$0(orcid)0000-0003-2993-5478$$aMartín, C.$$uUniversidad de Zaragoza
000095351 7102_ $$11011$$2630$$aUniversidad de Zaragoza$$bDpto. Microb.Ped.Radio.Sal.Pú.$$cÁrea Microbiología
000095351 7102_ $$11003$$2443$$aUniversidad de Zaragoza$$bDpto. Anatom.Histolog.Humanas$$cArea Histología
000095351 7102_ $$11009$$2773$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Sanidad Animal
000095351 7102_ $$11011$$2X$$aUniversidad de Zaragoza$$bDpto. Microb.Ped.Radio.Sal.Pú.$$cProy. investigación HQA
000095351 773__ $$g11 (2020), 1339 [15 pp.]$$pFront. microbiol.$$tFrontiers in Microbiology$$x1664-302X
000095351 8564_ $$s2717455$$uhttps://zaguan.unizar.es/record/95351/files/texto_completo.pdf$$yVersión publicada
000095351 8564_ $$s458290$$uhttps://zaguan.unizar.es/record/95351/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000095351 909CO $$ooai:zaguan.unizar.es:95351$$particulos$$pdriver
000095351 951__ $$a2024-01-05-10:13:52
000095351 980__ $$aARTICLE