95363 20210902121831.0 doi 10.3390/ma13143087 sideral 119162 ART-2020-119162 eng Smaida, R. Potential implantable nanofibrous biomaterials combined with stem cells for subchondral bone regeneration 2020 Access copy available to the general public Unrestricted The treatment of osteochondral defects remains a challenge. Four scaffolds were produced using Food and Drug Administration (FDA)-approved polymers to investigate their therapeutic potential for the regeneration of the osteochondral unit. Polycaprolactone (PCL) and poly(vinyl-pyrrolidone) (PVP) scaffolds were made by electrohydrodynamic techniques. Hydroxyapatite (HAp) and/or sodium hyaluronate (HA) can be then loaded to PCL nanofibers and/or PVP particles. The purpose of adding hydroxyapatite and sodium hyaluronate into PCL/PVP scaffolds is to increase the regenerative ability for subchondral bone and joint cartilage, respectively. Humanbone marrow-derived mesenchymal stem cells (hBM-MSCs) were seeded on these biomaterials. The biocompatibility of these biomaterials in vitro and in vivo, as well as their potential to support MSC differentiation under specific chondrogenic or osteogenic conditions, were evaluated. We show here that hBM-MSCs could proliferate and differentiate both in vitro and in vivo on these biomaterials. In addition, the PCL-HAp could effectively increase the mineralization and induce the differentiation of MSCs into osteoblasts in an osteogenic condition. These results indicate that PCL-HAp biomaterials combined with MSCs could be a beneficial candidate for subchondral bone regeneration. info:eu-repo/grantAgreement/ES/ISCIII-IIS/MS19-00092 info:eu-repo/semantics/openAccess by http://creativecommons.org/licenses/by/3.0/es/ 3.623 2020 METALLURGY & METALLURGICAL ENGINEERING 17 / 80 = 0.213 2020 Q1 T1 MATERIALS SCIENCE, MULTIDISCIPLINARY 152 / 333 = 0.456 2020 Q2 T2 PHYSICS, CONDENSED MATTER 27 / 69 = 0.391 2020 Q2 T2 PHYSICS, APPLIED 51 / 160 = 0.319 2020 Q2 T1 CHEMISTRY, PHYSICAL 79 / 162 = 0.488 2020 Q2 T2 0.682 2020 Materials Science (miscellaneous) 2020 Q2 Condensed Matter Physics 2020 Q2 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Pijnenburg, L. (orcid)0000-0002-2966-9088 Irusta, Silvia Universidad de Zaragoza Himawan, E. (orcid)0000-0003-2293-363X Mendoza, G. Universidad de Zaragoza Harmouch, E. Idoux-Gillet, Y. Kuchler-Bopp, S. Benkirane-Jessel, N. Hua, G. 5005 555 Universidad de Zaragoza Dpto. Ing.Quím.Tecnol.Med.Amb. Área Ingeniería Química 5005 790 Universidad de Zaragoza Dpto. Ing.Quím.Tecnol.Med.Amb. Área Tecnologi. Medio Ambiente 13, 14 (2020), 3087 [16 pp.] MATERIALS MATERIALS 1996-1944 9936056 http://zaguan.unizar.es/record/95363/files/texto_completo.pdf Versión publicada 468811 http://zaguan.unizar.es/record/95363/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:95363 articulos driver 2021-09-02-10:17:06 ARTICLE