000095442 001__ 95442 000095442 005__ 20210108084706.0 000095442 0247_ $$2doi$$a10.1039/c9nr06183e 000095442 0248_ $$2sideral$$a113565 000095442 037__ $$aART-2019-113565 000095442 041__ $$aeng 000095442 100__ $$0(orcid)0000-0001-8762-5457$$aSancho-Albero, María$$uUniversidad de Zaragoza 000095442 245__ $$aEfficient encapsulation of theranostic nanoparticles in cell-derived exosomes: leveraging the exosomal biogenesis pathway to obtain hollow gold nanoparticle-hybrids 000095442 260__ $$c2019 000095442 5060_ $$aAccess copy available to the general public$$fUnrestricted 000095442 5203_ $$aExosomes can be considered natural targeted delivery systems able to carry exogenous payloads, drugs or theranostic nanoparticles (NPs). This work aims to combine the therapeutic capabilities of hollow gold nanoparticles (HGNs) with the unique tumor targeting properties provided by exosomes. Here, we tested different methods to encapsulate HGNs (capable of absorbing light in the NIR region for selective thermal ablation) into murine melanoma cells derived exosomes (B16-F10-exos), including electroporation, passive loading by diffusion, thermal shock, sonication and saponin-assisted loading. These methods gave less than satisfactory results: although internalization of relatively large NPs into B16-F10-exos was achieved by almost all the physicochemical methods tested, only about 15% of the exosomes were loaded with NPs and several of those processes had a negative effect regarding the morphology and integrity of the loaded exosomes. In a different approach, B16-F10 cells were pre-incubated with PEGylated HGNs (PEG-HGNs) in an attempt to incorporate the NPs into the exosomal biogenesis pathway. The results were highly successful: exosomes recovered from the supernatant of the cell culture showed up to 50% of HGNs internalization. The obtained hybrid HGN-exosome vectors were characterized with a battery of techniques to make sure that internalization of HGNs did not affect exosome characteristics compared with other strategies. PEG-HGNs were released through the endosomal-exosome biogenesis pathway confirming that the isolated vesicles were exosomes. 000095442 536__ $$9This project has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No H2020 742684-CADENCE$$9info:eu-repo/grantAgreement/EC/H2020/742684/EU/Catalytic Dual-Function Devices Against Cancer/CADENCE$$9info:eu-repo/grantAgreement/EC/FP7/614715/EU/A Photo-triggered On-demand Drug Delivery System for Chronic Pain/NANOHEDONISM$$9info:eu-repo/grantAgreement/ES/UZ-DGA/T57-17R-P 000095442 540__ $$9info:eu-repo/semantics/openAccess$$aAll rights reserved$$uhttp://www.europeana.eu/rights/rr-f/ 000095442 590__ $$a6.895$$b2019 000095442 591__ $$aMATERIALS SCIENCE, MULTIDISCIPLINARY$$b50 / 314 = 0.159$$c2019$$dQ1$$eT1 000095442 591__ $$aNANOSCIENCE & NANOTECHNOLOGY$$b25 / 103 = 0.243$$c2019$$dQ1$$eT1 000095442 591__ $$aCHEMISTRY, MULTIDISCIPLINARY$$b28 / 177 = 0.158$$c2019$$dQ1$$eT1 000095442 591__ $$aPHYSICS, APPLIED$$b23 / 154 = 0.149$$c2019$$dQ1$$eT1 000095442 592__ $$a2.18$$b2019 000095442 593__ $$aNanoscience and Nanotechnology$$c2019$$dQ1 000095442 593__ $$aMaterials Science (miscellaneous)$$c2019$$dQ1 000095442 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/acceptedVersion 000095442 700__ $$0(orcid)0000-0003-4891-105X$$aEncabo-Berzosa, Maria del Mar 000095442 700__ $$aBeltrán-Visiedo, Manuel$$uUniversidad de Zaragoza 000095442 700__ $$aFernández-Messina, Lola 000095442 700__ $$0(orcid)0000-0002-6873-5244$$aSebastián, Víctor$$uUniversidad de Zaragoza 000095442 700__ $$aSánchez-Madrid, Francisco 000095442 700__ $$0(orcid)0000-0003-3165-0156$$aArruebo, Manuel$$uUniversidad de Zaragoza 000095442 700__ $$0(orcid)0000-0002-8701-9745$$aSantamaría, Jesús$$uUniversidad de Zaragoza 000095442 700__ $$0(orcid)0000-0003-4160-9720$$aMartín-Duque, Pilar 000095442 7102_ $$15005$$2555$$aUniversidad de Zaragoza$$bDpto. Ing.Quím.Tecnol.Med.Amb.$$cÁrea Ingeniería Química 000095442 7102_ $$11002$$2050$$aUniversidad de Zaragoza$$bDpto. Bioq.Biolog.Mol. Celular$$cÁrea Biología Celular 000095442 773__ $$g11 (2019), 18825-18836$$pNanoscale$$tNanoscale$$x2040-3364 000095442 8564_ $$s6203745$$uhttps://zaguan.unizar.es/record/95442/files/texto_completo.pdf$$yPostprint 000095442 8564_ $$s224989$$uhttps://zaguan.unizar.es/record/95442/files/texto_completo.jpg?subformat=icon$$xicon$$yPostprint 000095442 909CO $$ooai:zaguan.unizar.es:95442$$particulos$$pdriver 000095442 951__ $$a2021-01-08-08:04:15 000095442 980__ $$aARTICLE