000095444 001__ 95444
000095444 005__ 20210902121739.0
000095444 0247_ $$2doi$$a10.3390/agronomy10091427
000095444 0248_ $$2sideral$$a119762
000095444 037__ $$aART-2020-119762
000095444 041__ $$aeng
000095444 100__ $$aBuzón Durán, Laura
000095444 245__ $$aAntifungal activity of chitosan oligomers-amino acid conjugate complexes against Fusarium culmorum in spelt (Triticum spelta L.)
000095444 260__ $$c2020
000095444 5060_ $$aAccess copy available to the general public$$fUnrestricted
000095444 5203_ $$aFusarium head blight (FHB) is a complex disease of cereals caused by Fusarium species, which causes severe damages in terms of yield quality and quantity worldwide, and which produces mycotoxin contamination, posing a serious threat to public health. In the study presented herein, the antifungal activity against Fusarium culmorum of chitosan oligomers (COS)–amino acid conjugate complexes was investigated both in vitro and in vivo. The amino acids assayed were cysteine, glycine, proline and tyrosine. In vitro tests showed an enhancement of mycelial growth inhibition, with EC50 and EC90 effective concentration values ranging from 320 to 948 µg·mL−1 and from 1107 to 1407 µg·mL−1 respectively, for the conjugate complexes, as a result of the synergistic behavior between COS and the amino acids, tentatively ascribed to enhanced cell membrane damage originating from lipid peroxidation. Tests on colonies showed a maximum percentage reduction in the number of colonies at 1500 µg·mL−1 concentration, while grain tests were found to inhibit fungal growth, reducing deoxynivalenol content by 89%. The formulation that showed the best performance, i.e., the conjugate complex based on COS and tyrosine, was further investigated in a small-scale field trial with artificially inoculated spelt (Triticum spelta L.), and as a seed treatment to inhibit fungal growth in spelt seedlings. The field experiment showed that the chosen formulation induced a decrease in disease severity, with a control efficacy of 83.5%, while the seed tests showed that the treatment did not affect the percentage of germination and resulted in a lower incidence of root rot caused by the pathogen, albeit with a lower control efficacy (50%). Consequently, the reported conjugate complexes hold enough promise for crop protection applications to deserve further examination in larger field trials, with other Fusarium spp. pathogens and/or Triticum species.
000095444 536__ $$9info:eu-repo/grantAgreement/ES/UZ/UZ2019-TEC-07
000095444 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000095444 590__ $$a3.417$$b2020
000095444 591__ $$aAGRONOMY$$b16 / 91 = 0.176$$c2020$$dQ1$$eT1
000095444 591__ $$aPLANT SCIENCES$$b57 / 235 = 0.243$$c2020$$dQ1$$eT1
000095444 592__ $$a0.706$$b2020
000095444 593__ $$aAgronomy and Crop Science$$c2020$$dQ1
000095444 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000095444 700__ $$aMartín Gil, Jesús
000095444 700__ $$aMarcos Robles, José Luis
000095444 700__ $$aFombellida Villafruela, Ángel
000095444 700__ $$aPérez Lebeña, Eduardo
000095444 700__ $$0(orcid)0000-0003-2713-2786$$aMartín Ramos, Pablo$$uUniversidad de Zaragoza
000095444 7102_ $$15011$$2500$$aUniversidad de Zaragoza$$bDpto. CC.Agrar.y Medio Natural$$cArea Ingeniería Agroforestal
000095444 773__ $$g10, 9 (2020), [16 pp.]$$pAgronomy$$tAGRONOMY-BASEL$$x2073-4395
000095444 8564_ $$s5840663$$uhttps://zaguan.unizar.es/record/95444/files/texto_completo.pdf$$yVersión publicada
000095444 8564_ $$s512414$$uhttps://zaguan.unizar.es/record/95444/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000095444 909CO $$ooai:zaguan.unizar.es:95444$$particulos$$pdriver
000095444 951__ $$a2021-09-02-09:41:06
000095444 980__ $$aARTICLE