000095503 001__ 95503
000095503 005__ 20230622083312.0
000095503 0247_ $$2doi$$a10.3390/microorganisms8081155
000095503 0248_ $$2sideral$$a119575
000095503 037__ $$aART-2020-119575
000095503 041__ $$aeng
000095503 100__ $$aRuiz-Ripa, L.
000095503 245__ $$aMechanisms of linezolid resistance among enterococci of clinical origin in Spain—detection of optrA-and cfr(D)-carrying E. faecalis
000095503 260__ $$c2020
000095503 5060_ $$aAccess copy available to the general public$$fUnrestricted
000095503 5203_ $$aThe mechanisms of linezolid resistance among 13 E. faecalis and 6 E. faecium isolates, recovered from six Spanish hospitals during 2017–2018, were investigated. The presence of acquired linezolid resistance genes and mutations in 23S rDNA and in genes encoding for ribosomal proteins was analyzed by PCR and amplicon sequencing. Moreover, the susceptibility to 18 antimicrobial agents was investigated, and the respective molecular background was elucidated by PCR-amplicon sequencing and whole genome sequencing. The transferability of the linezolid resistance genes was evaluated by filter-mating experiments. The optrA gene was detected in all 13 E. faecalis isolates; and one optrA-positive isolate also carried the recently described cfr(D) gene. Moreover, one E. faecalis isolate displayed the nucleotide mutation G2576T in the 23S rDNA. This mutation was also present in all six E. faecium isolates. All linezolid-resistant enterococci showed a multiresistance phenotype and harbored several antimicrobial resistance genes, as well as many virulence determinants. The fexA gene was located upstream of the optrA gene in 12 of the E. faecalis isolates. Moreover, an erm(A)-like gene was located downstream of optrA in two isolates recovered from the same hospital. The optrA gene was transferable in all but one E. faecalis isolates, in all cases along with the fexA gene. The cfr(D) gene was not transferable. The presence of optrA and mutations in the 23S rDNA are the main mechanisms of linezolid resistance among E. faecalis and E. faecium, respectively. We report the first description of the cfr(D) gene in E. faecalis. The presence of the optrA and cfr(D) genes in Spanish hospitals is a public health concern.
000095503 536__ $$9info:eu-repo/grantAgreement/ES/FEDER-AEI/SAT2016-76571-R
000095503 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000095503 590__ $$a4.128$$b2020
000095503 591__ $$aMICROBIOLOGY$$b52 / 136 = 0.382$$c2020$$dQ2$$eT2
000095503 592__ $$a0.857$$b2020
000095503 593__ $$aMicrobiology$$c2020$$dQ2
000095503 593__ $$aVirology$$c2020$$dQ2
000095503 593__ $$aMicrobiology (medical)$$c2020$$dQ2
000095503 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000095503 700__ $$aFeßler, A.T.
000095503 700__ $$aHanke, D.
000095503 700__ $$aEichhorn, I.
000095503 700__ $$aAzcona-Gutiérrez, J.M.
000095503 700__ $$aPérez-Moreno, M.O.
000095503 700__ $$0(orcid)0000-0002-9742-1463$$aSeral, C.$$uUniversidad de Zaragoza
000095503 700__ $$aAspiroz, C.
000095503 700__ $$aAlonso, C.A.
000095503 700__ $$aTorres, L.
000095503 700__ $$aAlós, J.I.
000095503 700__ $$aSchwarz, S.
000095503 700__ $$aTorres, C.
000095503 7102_ $$11011$$2630$$aUniversidad de Zaragoza$$bDpto. Microb.Ped.Radio.Sal.Pú.$$cÁrea Microbiología
000095503 773__ $$g8, 8 (2020), 1155 1-17$$pMicroorganisms$$tMicroorganisms$$x2076-2607
000095503 8564_ $$s660792$$uhttps://zaguan.unizar.es/record/95503/files/texto_completo.pdf$$yVersión publicada
000095503 8564_ $$s491587$$uhttps://zaguan.unizar.es/record/95503/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000095503 909CO $$ooai:zaguan.unizar.es:95503$$particulos$$pdriver
000095503 951__ $$a2023-06-21-15:00:26
000095503 980__ $$aARTICLE