95513 20250131161521.0 doi 10.3389/fbioe.2020.00857 sideral 119588 ART-2020-119588 eng Aguilar-Machado, Diederich Enzymatic Processes Triggered by PEF for Astaxanthin Extraction From Xanthophyllomyces dendrorhous 2020 Access copy available to the general public Unrestricted The aim of this study was to evaluate the potential of pulsed electric fields (PEF) to improve the extraction of the lipid-soluble astaxanthin from fresh biomass of a wild-type (CECT 11028) and mutant (ATCC 74219) Xanthophyllomyces dendrorhous strain using ethanol as solvent. Inactivation and propidium uptake studies revealed that inactivation is a good index for estimated the proportion of irreversible permeabilized cells when inactivation is higher than 70% in the two strains. Ethanol was ineffective for extracting carotenoids from the PEF-treated cells (20 kV/cm, 135 µs) of the two strains. However, after aqueous incubation of PEF-treated X. dendrorhous ATCC 74219 cells for 12 h, up to 2.4 ± 0.05 mg/g dried weight (d.w.) of carotenoids were extracted in ethanol. From total carotenoid extracted, around 84% corresponded to all-trans astaxanthin. The detection and quantification of esterase activity in the supernatant and the relationship between the percentage of esterase activity quantified and the amount of carotenoids extracted indicate that the extraction of astaxanthin was mediated by enzymatic esterase activity triggered by PEF during incubation. On the other hand, the formation of a large lipid globule into the cytoplasm of PEF-treated X. dendrorhous CECT 11028 cells during aqueous incubation prevented carotenoid extraction. The process developed in this investigation represents a more sustainable and greener method that those previously used for extracting astaxanthin from yeast. info:eu-repo/semantics/openAccess by http://creativecommons.org/licenses/by/3.0/es/ 5.89 2020 MULTIDISCIPLINARY SCIENCES 11 / 71 = 0.155 2020 Q1 T1 1.081 2020 Bioengineering 2020 Q1 Histology 2020 Q1 Biotechnology 2020 Q1 Biomedical Engineering 2020 Q1 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Delso, Carlota Universidad de Zaragoza (orcid)0000-0003-4744-8649 Martinez, Juan Manuel (orcid)0000-0002-9337-417X Morales-Oyervides, Lourdes Montañez, Julio Raso, Javier Universidad de Zaragoza (orcid)0000-0003-3957-9091 2008 780 Universidad de Zaragoza Dpto. Produc.Animal Cienc.Ali. Área Tecnología de Alimentos 8 (2020), 857 1-12 Front. Bioeng. Biotechnol. Frontiers in Bioengineering and Biotechnology 2296-4185 4442647 http://zaguan.unizar.es/record/95513/files/texto_completo.pdf Versión publicada 487549 http://zaguan.unizar.es/record/95513/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:95513 articulos driver 2025-01-31-16:13:23 ARTICLE