95537 20211216131132.0 doi 10.1016/j.talanta.2019.120392 sideral 114709 ART-2020-114709 eng Navarro, Jesús Analytical possibilities of Putrescine and Cadaverine enzymatic colorimetric determination in tuna based on diamine oxidase: A critical study of the use of ABTS 2020 Access copy available to the general public Unrestricted The joint determination of putrescine (Put) and cadaverine (Cad) in the presence of other biogenic amines is studied using their enzymatic reaction with diamine oxidase (DAO). Three alternative methods are studied based on the intrinsic colorimetric properties of DAO or horseradish peroxidase (HRP), and the use of 2, 2'-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) colorimetric reagent, respectively. In this last case an in-depth study is carried out in order to explain and solve some drawbacks usually associated with the use of this reagent (especially interferences, interaction with enzymes and instability), and to propose new analytical methodologies which this reagent allows to achieve (transient signal and the use of the violet species). Finally, the method has been applied to Put + Cad determination in a tuna sample without interference of other biogenic amines. The result has been compared with that obtained using a method based on HPLC-MS, which has allowed the new methodology to be validated. info:eu-repo/grantAgreement/ES/DGA/E25-17R info:eu-repo/grantAgreement/ES/MINECO/CTQ2016-76846-R info:eu-repo/semantics/openAccess by-nc-nd http://creativecommons.org/licenses/by-nc-nd/3.0/es/ 6.057 2020 CHEMISTRY, ANALYTICAL 12 / 83 = 0.145 2020 Q1 T1 1.181 2020 Analytical Chemistry 2020 Q1 Spectroscopy 2020 Q1 Biochemistry 2020 Q1 info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion Sanz-Vicente, Isabel Universidad de Zaragoza (orcid)0000-0002-3906-4576 Lozano, Rebeca de Marcos, Susana Universidad de Zaragoza (orcid)0000-0002-7902-6005 Galbán, Javier Universidad de Zaragoza (orcid)0000-0002-8973-5104 2009 750 Universidad de Zaragoza Dpto. Química Analítica Área Química Analítica 208 (2020), 120392 1-8 Talanta TALANTA 0039-9140 1222880 http://zaguan.unizar.es/record/95537/files/texto_completo.pdf Postprint 448342 http://zaguan.unizar.es/record/95537/files/texto_completo.jpg?subformat=icon icon Postprint https://www.scopus.com/inward/record.uri?eid=2-s2.0-85072765676&doi=10.1016%2fj.talanta.2019.120392&partnerID=40&md5=8d8955d8084cb7042c6d523fcb0329b0 Texto completo de la revista oai:zaguan.unizar.es:95537 articulos driver 2021-12-16-13:02:14 ARTICLE