95564 20250319143307.0 doi 10.3390/microorganisms8060937 sideral 119706 ART-2020-119706 eng Berdejo, D. Universidad de Zaragoza (orcid)0000-0001-5053-8309 Genetic Variants and Phenotypic Characteristics ofSalmonellaTyphimurium-Resistant Mutants after Exposure to Carvacrol 2020 Access copy available to the general public Unrestricted The emergence of antimicrobial resistance has raised questions about the safety of essential oils and their individual constituents as food preservatives and as disinfection agents. Further research is required to understand how and under what conditions stable genotypic resistance might occur in food pathogens. Evolution experiments onSalmonellaTyphimurium cyclically exposed to sublethal and lethal doses of carvacrol permitted the isolation of SeSCar and SeLCar strains, respectively. Both evolved strains showed a significant increase in carvacrol resistance, assessed by minimum inhibitory and bactericidal concentrations, the study of growth kinetics in the presence of carvacrol, and the evaluation of survival under lethal conditions. Moreover, antibiotic susceptibility tests revealed a development of SeLCar resistance to a wide range of antibiotics. Whole genome sequencing allowed the identification of single nucleotide variations in transcriptional regulators of oxidative stress-response:yfhPin SeSCar andsoxRin SeLCar, which could be responsible for the increased resistance by improving the response to carvacrol and preventing its accumulation inside the cell. This study demonstrates the emergence of S. Typhimurium-resistant mutants against carvacrol, which might pose a risk to food safety and should therefore be considered in the design of food preservation strategies, or of cleaning and disinfection treatments. info:eu-repo/grantAgreement/ES/AEI-ESF-DGA/PGC2018-093789-B-I00 info:eu-repo/semantics/openAccess by http://creativecommons.org/licenses/by/3.0/es/ 4.128 2020 MICROBIOLOGY 52 / 135 = 0.385 2020 Q2 T2 0.857 2020 Microbiology 2020 Q2 Virology 2020 Q2 Microbiology (medical) 2020 Q2 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Merino, N. Universidad de Zaragoza (orcid)0000-0003-4774-0973 Pagan, E. Universidad de Zaragoza (orcid)0000-0003-3752-4660 Garcia-Gonzalo, D. Universidad de Zaragoza (orcid)0000-0002-7629-8101 Pagan, R. Universidad de Zaragoza (orcid)0000-0002-0238-6328 2008 780 Universidad de Zaragoza Dpto. Produc.Animal Cienc.Ali. Área Tecnología de Alimentos 8, 6 (2020), 937 [22 pp] Microorganisms Microorganisms 2076-2607 873681 http://zaguan.unizar.es/record/95564/files/texto_completo.pdf Versión publicada 491000 http://zaguan.unizar.es/record/95564/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:95564 articulos driver 2025-03-19-14:32:09 ARTICLE