000095607 001__ 95607
000095607 005__ 20230914083259.0
000095607 0247_ $$2doi$$a10.3390/biology9080207
000095607 0248_ $$2sideral$$a119735
000095607 037__ $$aART-2020-119735
000095607 041__ $$aeng
000095607 100__ $$0(orcid)0000-0001-5316-1703$$aYaniz, J.$$uUniversidad de Zaragoza
000095607 245__ $$aExpanding the Limits of Computer-Assisted Sperm Analysis through the Development of Open Software
000095607 260__ $$c2020
000095607 5060_ $$aAccess copy available to the general public$$fUnrestricted
000095607 5203_ $$aComputer assisted sperm analysis (CASA) systems can reduce errors occurring in manual analysis. However, commercial CASA systems are frequently not applicable at the forefront of challenging research endeavors. The development of open source software may offer important solutions for researchers working in related areas. Here, we present an example of this, with the development of three new modules for the OpenCASA software (hosted at Github). The first is theChemotactic Sperm Accumulation Module, a powerful tool for studying sperm chemotactic behavior, analyzing the sperm accumulation in the direct vicinity of the stimuli. This module was validated by comparing fish sperm accumulation, with or without the influence of an attractant. The analysis clearly indicated cell accumulation in the treatment group, while the distribution of sperm was random in the control group. The second is the Sperm Functionality Module, based on the ability to recognize five sperm subpopulations according to their fluorescence patterns associated with the plasma membrane and acrosomal status. The last module is the Sperm Concentration Module, which expands the utilities of OpenCASA. These last two modules were validated, using bull sperm, by comparing them with visual counting by an observer. A high level of correlation was achieved in almost all the data, and a good agreement between both methods was obtained. With these newly developed modules, OpenCASA is consolidated as a powerful free and open-source tool that allowsdifferent aspects of sperm quality to be evaluated, with many potential applications for researchers.
000095607 536__ $$9info:eu-repo/grantAgreement/ES/MINECO/BES-2017-079979$$9info:eu-repo/grantAgreement/ES/MINECO-AEI-ERDF/CICYT-PID2019-105660RB-C21$$9info:eu-repo/grantAgreement/ES/MINECO-AEI-ERDF/CICYT-AGL-2017-85030-R$$9info:eu-repo/grantAgreement/ES/MINECO-AEI-ERDF/CICYT-AGL2017-83799-R$$9info:eu-repo/grantAgreement/ES/DGA-FEDER/Construyendo Europa desde Aragón$$9info:eu-repo/grantAgreement/ES/DGA-ESF/T58-20R$$9info:eu-repo/grantAgreement/ES/DGA-ESF/A07-17R
000095607 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000095607 590__ $$a5.079$$b2020
000095607 591__ $$aBIOLOGY$$b16 / 93 = 0.172$$c2020$$dQ1$$eT1
000095607 592__ $$a1.731$$b2020
000095607 593__ $$aBiochemistry, Genetics and Molecular Biology (miscellaneous)$$c2020$$dQ1
000095607 593__ $$aAgricultural and Biological Sciences (miscellaneous)$$c2020$$dQ1
000095607 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000095607 700__ $$0(orcid)0000-0003-0389-2422$$aAlquezar-Baeta, C.$$uUniversidad de Zaragoza
000095607 700__ $$aYague-Martinez, J.
000095607 700__ $$0(orcid)0000-0003-4164-5078$$aAlastruey-Benede, J.$$uUniversidad de Zaragoza
000095607 700__ $$0(orcid)0000-0003-1228-535X$$aPalacin, I.$$uUniversidad de Zaragoza
000095607 700__ $$aBoryshpolets, S.
000095607 700__ $$aKholodnyy, V.
000095607 700__ $$aGadelha, H.
000095607 700__ $$0(orcid)0000-0002-2312-6402$$aPerez-Pe, R.$$uUniversidad de Zaragoza
000095607 7102_ $$12006$$2440$$aUniversidad de Zaragoza$$bDpto. Matemáticas$$cÁrea Geometría y Topología
000095607 7102_ $$11002$$2819$$aUniversidad de Zaragoza$$bDpto. Bioq.Biolog.Mol. Celular$$cÁrea Zoología
000095607 7102_ $$15007$$2035$$aUniversidad de Zaragoza$$bDpto. Informát.Ingenie.Sistms.$$cÁrea Arquit.Tecnología Comput.
000095607 7102_ $$12008$$2700$$aUniversidad de Zaragoza$$bDpto. Produc.Animal Cienc.Ali.$$cÁrea Producción Animal
000095607 773__ $$g9, 8 (2020), 207 [16 pp]$$tBiology$$x2079-7737
000095607 8564_ $$s741437$$uhttps://zaguan.unizar.es/record/95607/files/texto_completo.pdf$$yVersión publicada
000095607 8564_ $$s498168$$uhttps://zaguan.unizar.es/record/95607/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000095607 909CO $$ooai:zaguan.unizar.es:95607$$particulos$$pdriver
000095607 951__ $$a2023-09-13-10:52:25
000095607 980__ $$aARTICLE