95737 20230519145347.0 doi 10.1139/bcb-2020-0121 sideral 120177 ART-2021-120177 eng Buey, Berta (orcid)0000-0002-8467-0356 Comparative effect of bovine buttermilk, whey, and lactoferrin on the innate immunity receptors and oxidative status of intestinal epithelial cells 2021 Access copy available to the general public Unrestricted Milk contains active molecules with important functional properties as the defensive proteins; among them are the whey protein lactoferrin and proteins of the milk fat globule membrane (MFGM) present in buttermilk. The aim of this study has been to investigate the effect of lactoferrin, whey and buttermilk as modulators of intestinal innate immunity and oxidative stress on intestinal epithelial cells, to evaluate its potential use for the development of functional foods. Innate immune Toll-like receptors (TLR2, TLR4, and TLR9) mRNA expression, lipid peroxidation (MDA+4-HDA) and protein carbonyl levels were analyzed in enterocyte-like Caco-2/TC7 cells treated for 24 hours with different concentrations of lactoferrin, whey or buttermilk. None of the substances analyzed caused oxidative damage; however, whey significantly decreased the levels of lipid peroxidation. Furthermore, both lactoferrin and whey were able to reduce the oxidative stress induced by lipopolysaccharide. Respect to TLR receptors, lactoferrin, whey and buttermilk specifically altered the expression of TLR2, TLR4 and TLR9 receptors, with a strong decrease in TLR4 expression. These results suggest that lactoferrin, whey and buttermilk could be interesting potential ingredients for functional foods as they seem to modulate oxidative stress and inflammatory response induced by TLRs activation. info:eu-repo/grantAgreement/ES/DGA-FEDER/A02-17R info:eu-repo/grantAgreement/ES/MINECO/AGL2017-82987-R info:eu-repo/semantics/openAccess All rights reserved http://www.europeana.eu/rights/rr-f/ 3.73 2021 CELL BIOLOGY 132 / 195 = 0.677 2021 Q3 T3 BIOCHEMISTRY & MOLECULAR BIOLOGY 176 / 297 = 0.593 2021 Q3 T2 0.694 2021 Cell Biology 2021 Q2 Biochemistry 2021 Q2 6.0 2021 info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion Bellés, Andrea Universidad de Zaragoza (orcid)0000-0002-6546-6149 Latorre, Eva Universidad de Zaragoza (orcid)0000-0002-5797-3909 Abad, Inés Universidad de Zaragoza Pérez, María Dolores Universidad de Zaragoza (orcid)0000-0003-2555-8425 Grasa, Laura Universidad de Zaragoza (orcid)0000-0002-5306-9365 Mesonero, Jose Emilio Universidad de Zaragoza (orcid)0000-0003-4758-3998 Sánchez, Lourdes Universidad de Zaragoza (orcid)0000-0001-5964-823X 1012 410 Universidad de Zaragoza Dpto. Farmac.Fisiol.y Med.L.F. Área Fisiología 1002 050 Universidad de Zaragoza Dpto. Bioq.Biolog.Mol. Celular Área Biología Celular 2008 780 Universidad de Zaragoza Dpto. Produc.Animal Cienc.Ali. Área Tecnología de Alimentos 99, 1 (2021), 2020-0121 Biochem. cell. biol. BIOCHEMISTRY AND CELL BIOLOGY-BIOCHIMIE ET BIOLOGIE CELLULAIRE 0829-8211 2474808 http://zaguan.unizar.es/record/95737/files/texto_completo.pdf Postprint 178957 http://zaguan.unizar.es/record/95737/files/texto_completo.jpg?subformat=icon icon Postprint oai:zaguan.unizar.es:95737 articulos driver 2023-05-18-13:22:23 ARTICLE