000095746 001__ 95746
000095746 005__ 20210902121815.0
000095746 0247_ $$2doi$$a10.1038/s41598-020-71985-w
000095746 0248_ $$2sideral$$a120183
000095746 037__ $$aART-2020-120183
000095746 041__ $$aeng
000095746 100__ $$aMorcillo, M.
000095746 245__ $$aEffect of elicitors on holm oak somatic embryo development and efficacy inducing tolerance to Phytophthora cinnamomi
000095746 260__ $$c2020
000095746 5060_ $$aAccess copy available to the general public$$fUnrestricted
000095746 5203_ $$aHolm oak trees (Quercus ilex L.) mortality is increasing worryingly in the Mediterranean area in the last years. To a large degree this mortality is caused by the oomycete Phytophthora spp., which is responsible for forest decline and dieback in evergreen oak forest areas of the southwestern Iberian Peninsula. This study is based on the possibility of applying chemical elicitors or filtered oomycete extracts to holm oak somatic embryos (SE) in order to induce epigenetic memory, priming, that may increase tolerance to the pathogen in future infections. To this end, we first examined the effect of priming treatments on SE development and its oxidative stress state, to avoid elicitors that may cause damage to embryogenic tissues. Both, the sterile oomycete extracts and the chemical elicitor methyl jasmonate (MeJA) did not produce any detrimental effect on SE growth and development, unlike the elicitors benzothiadiazole (BTH) and p-aminobenzoic acid (PABA) that reduced the relative weight gain and resulted in necrotic and deformed SE when were applied at high concentrations (25 µM BTH or 50 µM PABA) in accordance with their high malondialdehyde content. No significant differences among elicitation treatments were found in dual culture bioassays, although those SEs elicited with 50 µM MeJA increased H2O2 production after challenged against active oomycete indicating the activation of stress response. Since this elicitation treatment did not produce any adverse effect in the embryogenic process we suggest that could be used in further priming experiments to produce holm oak plants adapted to biotic stress.
000095746 536__ $$9info:eu-repo/grantAgreement/ES/MICINN-FEDER/AGL2013-47400-C4-04-R$$9info:eu-repo/grantAgreement/ES/MICINN-FEDER/AGL2016-76143-C4-01-R
000095746 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000095746 590__ $$a4.379$$b2020
000095746 591__ $$aMULTIDISCIPLINARY SCIENCES$$b17 / 73 = 0.233$$c2020$$dQ1$$eT1
000095746 592__ $$a1.24$$b2020
000095746 593__ $$aMultidisciplinary$$c2020$$dQ1
000095746 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000095746 700__ $$0(orcid)0000-0003-0582-5418$$aSales, E.$$uUniversidad de Zaragoza
000095746 700__ $$aPonce, L.
000095746 700__ $$aGuillén, A.
000095746 700__ $$aSegura, J.
000095746 700__ $$aArrillaga, I.
000095746 7102_ $$15011$$2705$$aUniversidad de Zaragoza$$bDpto. CC.Agrar.y Medio Natural$$cÁrea Producción Vegetal
000095746 773__ $$g10, 1 (2020), 15166 [10 pp]$$pSci. rep.$$tScientific Reports$$x2045-2322
000095746 8564_ $$s1574856$$uhttps://zaguan.unizar.es/record/95746/files/texto_completo.pdf$$yVersión publicada
000095746 8564_ $$s343152$$uhttps://zaguan.unizar.es/record/95746/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000095746 909CO $$ooai:zaguan.unizar.es:95746$$particulos$$pdriver
000095746 951__ $$a2021-09-02-10:04:18
000095746 980__ $$aARTICLE