000095766 001__ 95766
000095766 005__ 20230921135433.0
000095766 0247_ $$2doi$$a10.3390/biom10091334
000095766 0248_ $$2sideral$$a120203
000095766 037__ $$aART-2020-120203
000095766 041__ $$aeng
000095766 100__ $$aOliver-Gelabert, A.$$uUniversidad de Zaragoza
000095766 245__ $$aAutomatic quantification of cardiomyocyte dimensions and connexin 43 lateralization in fluorescence images
000095766 260__ $$c2020
000095766 5060_ $$aAccess copy available to the general public$$fUnrestricted
000095766 5203_ $$aCardiomyocytes’ geometry and connexin 43 (CX43) amount and distribution are structural features that play a pivotal role in electrical conduction. Their quantitative assessment is of high interest in the study of arrhythmias, but it is usually hampered by the lack of automatic tools. In this work, we propose a software algorithm (Myocyte Automatic Retrieval and Tissue Analyzer, MARTA) to automatically detect myocytes from fluorescent microscopy images of cardiac tissue, measure their morphological features and evaluate the expression of CX43 and its degree of lateralization. The proposed software is based on the generation of cell masks, contouring of individual cells, enclosing of cells in minimum area rectangles and splitting of these rectangles into end-to-end and middle compartments to estimate CX43 lateral-to-total ratio. Application to human ventricular tissue images shows that mean differences between automatic and manual methods in terms of cardiomyocyte length and width are below 4 µm. The percentage of lateral CX43 also agrees between automatic and manual evaluation, with the interquartile range approximately covering from 3% to 30% in both cases. MARTA is not limited by fiber orientation and has an optimized speed by using contour filtering, which makes it run hundreds of times faster than a trained expert. Developed for CX43 studies in the left ventricle, MARTA is a flexible tool applicable to morphometric and lateralization studies of other markers in any heart chamber or even skeletal muscle. This open-access software is available online.
000095766 536__ $$9info:eu-repo/grantAgreement/ES/MICIU/PID2019-105674RB-I00$$9info:eu-repo/grantAgreement/EUR/ERC-2014-StG-638284$$9info:eu-repo/grantAgreement/ES/DGA/LMP124-18$$9info:eu-repo/grantAgreement/ES/DGA-FSE/T39-20R-BSICoS group$$9info:eu-repo/grantAgreement/ES/DGA/C150-2016
000095766 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000095766 590__ $$a4.879$$b2020
000095766 591__ $$aBIOCHEMISTRY & MOLECULAR BIOLOGY$$b96 / 296 = 0.324$$c2020$$dQ2$$eT1
000095766 592__ $$a1.125$$b2020
000095766 593__ $$aMolecular Biology$$c2020$$dQ2
000095766 593__ $$aBiochemistry$$c2020$$dQ2
000095766 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000095766 700__ $$0(orcid)0000-0002-2954-1068$$aGarcía-Mendívil, L.$$uUniversidad de Zaragoza
000095766 700__ $$aVallejo-Gil, J.M.
000095766 700__ $$aFresneda-Roldán, P.C.
000095766 700__ $$aAndelová, K.
000095766 700__ $$aFañanás-Mastral, J.
000095766 700__ $$aVázquez-Sancho, M.
000095766 700__ $$0(orcid)0000-0001-7482-2582$$aMatamala-Adell, M.$$uUniversidad de Zaragoza
000095766 700__ $$aSorribas-Berjón, F.
000095766 700__ $$0(orcid)0000-0002-2225-308X$$aBallester-Cuenca, C.$$uUniversidad de Zaragoza
000095766 700__ $$aTribulova, N.
000095766 700__ $$0(orcid)0000-0003-3982-1263$$aOrdovás, L.
000095766 700__ $$aDiez, E.R.
000095766 700__ $$0(orcid)0000-0002-1960-407X$$aPueyo, E.$$uUniversidad de Zaragoza
000095766 7102_ $$11013$$2090$$aUniversidad de Zaragoza$$bDpto. Cirugía$$cÁrea Cirugía
000095766 7102_ $$15008$$2800$$aUniversidad de Zaragoza$$bDpto. Ingeniería Electrón.Com.$$cÁrea Teoría Señal y Comunicac.
000095766 773__ $$g10, 9 (2020), 1334 [25 pp.]$$tBiomolecules$$x2218-273X
000095766 8564_ $$s1816246$$uhttps://zaguan.unizar.es/record/95766/files/texto_completo.pdf$$yVersión publicada
000095766 8564_ $$s506690$$uhttps://zaguan.unizar.es/record/95766/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000095766 909CO $$ooai:zaguan.unizar.es:95766$$particulos$$pdriver
000095766 951__ $$a2023-09-21-13:30:32
000095766 980__ $$aARTICLE