000095767 001__ 95767
000095767 005__ 20210902121733.0
000095767 0247_ $$2doi$$a10.3390/ph13090239
000095767 0248_ $$2sideral$$a120204
000095767 037__ $$aART-2020-120204
000095767 041__ $$aeng
000095767 100__ $$0(orcid)0000-0001-5053-8309$$aBerdejo, D.$$uUniversidad de Zaragoza
000095767 245__ $$aIncubation with a complex orange essential oil leads to evolved mutants with increased resistance and tolerance
000095767 260__ $$c2020
000095767 5060_ $$aAccess copy available to the general public$$fUnrestricted
000095767 5203_ $$aEmergence of strains with increased resistance/tolerance to natural antimicrobials was evidenced after cyclic exposure to carvacrol, citral, and (+)-limonene oxide. However, no previous studies have reported the development of resistance and tolerance to complex essential oils (EOs). This study seeks to evaluate the occurrence of Staphylococcus aureus strains resistant and tolerant to a complex orange essential oil (OEO) after prolonged cyclic treatments at low concentrations. Phenotypic characterization of evolved strains revealed an increase of minimum inhibitory and bactericidal concentration for OEO, a better growth fitness in presence of OEO, and an enhanced survival to lethal treatments, compared to wild-type strain. However, no significant differences (p > 0.05) in cross-resistance to antibiotics were observed. Mutations in hepT and accA in evolved strains highlight the important role of oxidative stress in the cell response to OEO, as well as the relevance of the cell membrane in the cell response to these natural antimicrobials. This study demonstrates the emergence of S. aureus strains that are resistant and tolerant to EO (Citrus sinensis). This phenomenon should be taken into account to assure the efficacy of natural antimicrobials in the design of food preservation strategies, in cleaning and disinfection protocols, and in clinical applications against resistant bacteria.
000095767 536__ $$9info:eu-repo/grantAgreement/ES/AEI-ESF-DGA/PGC2018-093789-B-I00
000095767 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000095767 590__ $$a5.863$$b2020
000095767 591__ $$aPHARMACOLOGY & PHARMACY$$b38 / 275 = 0.138$$c2020$$dQ1$$eT1
000095767 591__ $$aCHEMISTRY, MEDICINAL$$b9 / 63 = 0.143$$c2020$$dQ1$$eT1
000095767 592__ $$a1.294$$b2020
000095767 593__ $$aDrug Discovery$$c2020$$dQ1
000095767 593__ $$aPharmaceutical Science$$c2020$$dQ1
000095767 593__ $$aMolecular Medicine$$c2020$$dQ1
000095767 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000095767 700__ $$0(orcid)0000-0003-3752-4660$$aPagán, E.$$uUniversidad de Zaragoza
000095767 700__ $$0(orcid)0000-0003-4460-3543$$aMerino, N.$$uUniversidad de Zaragoza
000095767 700__ $$0(orcid)0000-0002-0238-6328$$aPagán, R.$$uUniversidad de Zaragoza
000095767 700__ $$0(orcid)0000-0002-7629-8101$$aGarcía-Gonzalo, D.$$uUniversidad de Zaragoza
000095767 7102_ $$12008$$2780$$aUniversidad de Zaragoza$$bDpto. Produc.Animal Cienc.Ali.$$cÁrea Tecnología de Alimentos
000095767 773__ $$g13, 9 (2020), 239 [1-17]$$pPharmaceuticals$$tPharmaceuticals$$x1424-8247
000095767 8564_ $$s598687$$uhttps://zaguan.unizar.es/record/95767/files/texto_completo.pdf$$yVersión publicada
000095767 8564_ $$s514440$$uhttps://zaguan.unizar.es/record/95767/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000095767 909CO $$ooai:zaguan.unizar.es:95767$$particulos$$pdriver
000095767 951__ $$a2021-09-02-09:36:44
000095767 980__ $$aARTICLE