000095775 001__ 95775
000095775 005__ 20230914083302.0
000095775 0247_ $$2doi$$a10.3390/biology9090300
000095775 0248_ $$2sideral$$a120213
000095775 037__ $$aART-2020-120213
000095775 041__ $$aeng
000095775 100__ $$aSadeghi, S.
000095775 245__ $$aEffect of sperm concentration and storage temperature on goat spermatozoa during liquid storage
000095775 260__ $$c2020
000095775 5060_ $$aAccess copy available to the general public$$fUnrestricted
000095775 5203_ $$aThe use of cooled semen is relatively common in goats. There are a number of advantages of cooled semen doses, including easier handling of artificial insemination (AI) doses, transport, more AI doses per ejaculate, and higher fertility rates in comparison with frozen AI doses. However, cooled semen has a short shelf life. The objective of this study was to examine the effect of temperature and sperm concentration on the in vitro sperm quality during liquid storage for 48 h, including sperm motility and kinetics, response to oxidation, mitochondrial membrane potential (MMP) and DNA fragmentation in goats. Three experiments were performed. In the first, the effects of liquid preservation of semen at different temperatures (5 °C or 17 °C), durations (0, 24 and 48 h) and sperm concentrations (250 × 106 sperm/mL (1:2 dilution rate), 166.7 × 106 sperm/mL (1:3 dilution rate) or 50 × 106 sperm/mL (1:10 dilution rate)) on sperm motility and kinetics were studied. In the second experiment, the effect of temperature, sperm washing and concentration on sperm motility and DNA fragmentation was studied. Finally, the effect of sperm concentration and duration of storage at 5 °C on sperm motility, response to oxidative stress and MMP was examined. We found that refrigerated liquid storage of goat sperm impaired sperm quality, such as motility, MMP and response to oxidation, as storage time increased; however, sperm DNA fragmentation index was not significantly affected. Liquid storage at 5 °C preserved higher total motility than at 17 °C. Moreover, we observed that the reduction of sperm concentration below 500 × 106 sperm/mL did not seem to improve the quality of spermatozoa conserved in milk-based extender in the conditions tested
000095775 536__ $$9info:eu-repo/grantAgreement/ES/DGA-ESF/A07-17R$$9info:eu-repo/grantAgreement/ES/INIA/RTA2013-00107-C03-03$$9info:eu-repo/grantAgreement/ES/MINECO/AGL2017-85030-R
000095775 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000095775 590__ $$a5.079$$b2020
000095775 591__ $$aBIOLOGY$$b16 / 93 = 0.172$$c2020$$dQ1$$eT1
000095775 592__ $$a1.731$$b2020
000095775 593__ $$aBiochemistry, Genetics and Molecular Biology (miscellaneous)$$c2020$$dQ1
000095775 593__ $$aAgricultural and Biological Sciences (miscellaneous)$$c2020$$dQ1
000095775 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000095775 700__ $$aGallego, R.D.
000095775 700__ $$aGarcía-Colomer, B.
000095775 700__ $$aGómez, E.A.
000095775 700__ $$0(orcid)0000-0001-5316-1703$$aYániz, J.L.$$uUniversidad de Zaragoza
000095775 700__ $$aGosálvez, J.
000095775 700__ $$aLópez-Fernández, C.
000095775 700__ $$aSilvestre, M.A.
000095775 7102_ $$12008$$2700$$aUniversidad de Zaragoza$$bDpto. Produc.Animal Cienc.Ali.$$cÁrea Producción Animal
000095775 773__ $$g9, 9 (2020), 300 [13 pp.]$$tBiology$$x2079-7737
000095775 8564_ $$s550789$$uhttps://zaguan.unizar.es/record/95775/files/texto_completo.pdf$$yVersión publicada
000095775 8564_ $$s513677$$uhttps://zaguan.unizar.es/record/95775/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000095775 909CO $$ooai:zaguan.unizar.es:95775$$particulos$$pdriver
000095775 951__ $$a2023-09-13-10:53:27
000095775 980__ $$aARTICLE