000095838 001__ 95838
000095838 005__ 20201021090935.0
000095838 0247_ $$2doi$$a10.1093/aob/mcz169
000095838 0248_ $$2sideral$$a116365
000095838 037__ $$aART-2019-116365
000095838 041__ $$aeng
000095838 100__ $$0(orcid)0000-0002-9012-8627$$aShiposha, Valeriia
000095838 245__ $$aMultiple founder events explain the genetic diversity and structure of the model allopolyploid grass Brachypodium hybridum in the Iberian Peninsula hotspot
000095838 260__ $$c2019
000095838 5060_ $$aAccess copy available to the general public$$fUnrestricted
000095838 5203_ $$aBackground and Aims: It is accepted that contemporary allopolyploid species have originated recurrently, but very few cases have been documented using multiple natural formations of the same species. To extend our knowledge, we have investigated the multiple origins, genetic variation and structure of the allotetraploid grass Brachypodium hybridum with respect to its progenitor diploid species B. distachyon (D genome) and B. stacei (S genome). For this, our primary focus is the Iberian Peninsula, an evolutionary hotspot for the genus Brachypodium. Methods: We analysed 342 B. hybridum individuals from 36 populations using ten nuclear SSR loci and two plastid loci. The B. hybridum genetic profiles were compared with those previously reported for B. stacei and B. distachyon. In addition, phylogenetic analysis of the plastid data was performed for a reduced subset of individuals. Key Results: The nuclear simple sequence repeat (SSR) genetic analysis detected medium to high genetic diversity, with a strong south-to-north genetic structure cline, and a high selfing rate in B. hybridum. Comparative genetic analysis showed a close relatedness of current B. hybridum D allelic profiles with those of B. distachyon, but a lack of similarity with those of B. stacei, suggesting another B. stacei source for the B. hybridum S alleles. Plastid analysis detected three different bidirectional allopolyploidization events: two involved distinct B. distachyon-like ancestors and one involved a B. stacei-like ancestor. The south-eastern Iberian Peninsula B. hybridum populations were more genetically diverse and could have originated from at least two hybridization events whereas north-eastern/north-western Iberian Peninsula B. hybridum populations were less diverse and may have derived from at least one hybridization event. Conclusions: The genetic and evolutionary evidence supports the plausible in situ origin of the south-eastern and northern Iberian Peninsula B. hybridum allopolyploids from their respective local B. distachyon and unknown B. stacei ancestors. The untapped multiple origins and genetic variation detected in these B. hybridum populations opens the way to future evolutionary analysis of allopolyploid formation and genomic dominance and expression in the B. hybridum–B. distachyon–B. stacei grass model complex.
000095838 536__ $$9info:eu-repo/grantAgreement/ES/MINECO/CGL2016-79790-P$$9info:eu-repo/grantAgreement/ES/UZ/UZ2016-TEC-02
000095838 540__ $$9info:eu-repo/semantics/openAccess$$aAll rights reserved$$uhttp://www.europeana.eu/rights/rr-f/
000095838 590__ $$a4.005$$b2019
000095838 591__ $$aPLANT SCIENCES$$b27 / 234 = 0.115$$c2019$$dQ1$$eT1
000095838 592__ $$a1.615$$b2019
000095838 593__ $$aPlant Science$$c2019$$dQ1
000095838 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/acceptedVersion
000095838 700__ $$aMarques, Isabel
000095838 700__ $$0(orcid)0000-0002-7734-8481$$aLópez-Alvarez, Diana
000095838 700__ $$aManzaneda, Antonio J
000095838 700__ $$aHernandez, Pilar
000095838 700__ $$aOlonova, Marina
000095838 700__ $$0(orcid)0000-0001-7793-5259$$aCatalán, Pilar$$uUniversidad de Zaragoza
000095838 7102_ $$15011$$2063$$aUniversidad de Zaragoza$$bDpto. CC.Agrar.y Medio Natural$$cÁrea Botánica
000095838 773__ $$g125, 4 (2019), 625–638$$pAnn. bot.$$tAnnals of botany$$x1095-8290
000095838 8564_ $$s17101388$$uhttps://zaguan.unizar.es/record/95838/files/texto_completo.pdf$$yPostprint
000095838 8564_ $$s31886$$uhttps://zaguan.unizar.es/record/95838/files/texto_completo.jpg?subformat=icon$$xicon$$yPostprint
000095838 909CO $$ooai:zaguan.unizar.es:95838$$particulos$$pdriver
000095838 951__ $$a2020-10-21-08:24:46
000095838 980__ $$aARTICLE