000095848 001__ 95848
000095848 005__ 20201021181232.0
000095848 0247_ $$2doi$$a10.1371/journal.pone.0019909
000095848 0248_ $$2sideral$$a82078
000095848 037__ $$aART-2011-82078
000095848 041__ $$aeng
000095848 100__ $$0(orcid)0000-0003-3352-740X$$aFilali, Hicham
000095848 245__ $$aGene expression profiling and association with prion-related lesions in the medulla oblongata of symptomatic natural scrapie animals
000095848 260__ $$c2011
000095848 5060_ $$aAccess copy available to the general public$$fUnrestricted
000095848 5203_ $$aThe pathogenesis of natural scrapie and other prion diseases remains unclear. Examining transcriptome variations in infected versus control animals may highlight new genes potentially involved in some of the molecular mechanisms of prion-induced pathology. The aim of this work was to identify disease-associated alterations in the gene expression profiles of the caudal medulla oblongata (MO) in sheep presenting the symptomatic phase of natural scrapie. The gene expression patterns in the MO from 7 sheep that had been naturally infected with scrapie were compared with 6 controls using a Central Veterinary Institute (CVI) custom designed 4×44K microarray. The microarray consisted of a probe set on the previously sequenced ovine tissue library by CVI and was supplemented with all of the Ovis aries transcripts that are currently publicly available. Over 350 probe sets displayed greater than 2-fold changes in expression. We identified 148 genes from these probes, many of which encode proteins that are involved in the immune response, ion transport, cell adhesion, and transcription. Our results confirm previously published gene expression changes that were observed in murine models with induced scrapie. Moreover, we have identified new genes that exhibit differential expression in scrapie and could be involved in prion neuropathology. Finally, we have investigated the relationship between gene expression profiles and the appearance of the main scrapie-related lesions, including prion protein deposition, gliosis and spongiosis. In this context, the potential impacts of these gene expression changes in the MO on scrapie development are discussed.
000095848 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000095848 590__ $$a4.092$$b2011
000095848 591__ $$aBIOLOGY$$b12 / 85 = 0.141$$c2011$$dQ1$$eT1
000095848 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000095848 700__ $$0(orcid)0000-0001-6016-4726$$aMartín Burriel, Inmaculada$$uUniversidad de Zaragoza
000095848 700__ $$aHarders, Frank
000095848 700__ $$0(orcid)0000-0001-6256-5478$$aVarona, Luis$$uUniversidad de Zaragoza
000095848 700__ $$0(orcid)0000-0003-4796-1872$$aLyahyai, Jaber
000095848 700__ $$0(orcid)0000-0001-5105-6133$$aAcín, Cristina$$uUniversidad de Zaragoza
000095848 700__ $$0(orcid)0000-0001-5740-0185$$aZaragoza, Pilar$$uUniversidad de Zaragoza
000095848 700__ $$0(orcid)0000-0002-0935-7941$$aPumarola, Martí
000095848 700__ $$0(orcid)0000-0002-7173-7216$$aBadiola, Juan J$$uUniversidad de Zaragoza
000095848 700__ $$aBossers, Alex
000095848 700__ $$0(orcid)0000-0002-2746-3932$$aBolea, Rosa$$uUniversidad de Zaragoza
000095848 7102_ $$11001$$2420$$aUniversidad de Zaragoza$$bDpto. Anatom.,Embri.Genét.Ani.$$cÁrea Genética
000095848 7102_ $$11009$$2773$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Sanidad Animal
000095848 773__ $$g6, 5 (2011), e19909 [12 pp]$$pPLoS One$$tPloS one$$x1932-6203
000095848 8564_ $$s986431$$uhttps://zaguan.unizar.es/record/95848/files/texto_completo.pdf$$yVersión publicada
000095848 8564_ $$s40328$$uhttps://zaguan.unizar.es/record/95848/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000095848 909CO $$ooai:zaguan.unizar.es:95848$$particulos$$pdriver
000095848 951__ $$a2020-10-21-16:47:43
000095848 980__ $$aARTICLE