95851 20240122154814.0 doi 10.1155/2014/982639 sideral 87530 ART-2014-87530 eng Latorre, Eva Universidad de Zaragoza (orcid)0000-0002-5797-3909 IL-10 counteracts proinflammatory mediator evoked oxidative stress in Caco-2 cells. 2014 Access copy available to the general public Unrestricted Oxidative stress is thought to play a key role in the development of intestinal damage in intestinal inflammatory diseases. Several molecules are involved in the intestinal inflammation, either as pro- or anti-inflammatory factors;however, their effects on intestinal oxidative stress seem to be controversial. This work analyzes the contribution of pro- and anti-inflammatory molecules to the balance of oxidative damage in intestinal epithelial cells, as well as their effects on cellular antioxidant enzyme activity. With this purpose, the lipid and protein oxidation, together with the activity of catalase, superoxide dismutase, and glutathione peroxidase, were determined in the Caco-2 cells treated with serotonin, adenosine, melatonin, and TNF??, as proinflammatory factors, and IL-10, as an anti-inflammatory cytokine.The results have shown that all the proinflammatory factors assayed increased oxidative damage. In addition, these factors also inhibited the activity of antioxidant enzymes in the cells, except melatonin. In contrast, IL-10 did not alter these parameters but was able to reduce the prooxidant effects yielded by serotonin, adenosine, melatonin, or TNF??, in part by restoring the antioxidant enzymes activities. In summary, proinflammatory factors may induce oxidative damage in intestinal epithelial cells, whereas IL-10 seems to be able to restore the altered redox equilibrium in Caco-2 cells. info:eu-repo/grantAgreement/ES/DGA/ARAINF-012-2008 info:eu-repo/grantAgreement/ES/DGA/B022-13 info:eu-repo/grantAgreement/ES/DGA/B105-11 info:eu-repo/grantAgreement/ES/DGA-FSE/B61 info:eu-repo/grantAgreement/ES/MICINN-FEDER/BFU2010-18971 info:eu-repo/semantics/openAccess by http://creativecommons.org/licenses/by/3.0/es/ 3.236 2014 IMMUNOLOGY 58 / 147 = 0.395 2014 Q2 T2 CELL BIOLOGY 97 / 184 = 0.527 2014 Q3 T2 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Matheus, Nyurky (orcid)0000-0002-1605-0542 Layunta, Elena (orcid)0000-0001-5573-6144 Alcalde, Ana Isabel Universidad de Zaragoza (orcid)0000-0002-9935-927X Mesonero, José Emilio Universidad de Zaragoza (orcid)0000-0003-4758-3998 1005 410 Universidad de Zaragoza Dpto. Farmacología y Fisiolog. Área Fisiología 2014, 14 (2014), 982639 [6 pp] Mediat. inflamm. MEDIATORS OF INFLAMMATION 0962-9351 262730 http://zaguan.unizar.es/record/95851/files/texto_completo.pdf Versión publicada 32796 http://zaguan.unizar.es/record/95851/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:95851 articulos driver 2024-01-22-15:35:00 ARTICLE