000095868 001__ 95868
000095868 005__ 20210902121620.0
000095868 0247_ $$2doi$$a10.1038/s41598-019-57005-6
000095868 0248_ $$2sideral$$a116402
000095868 037__ $$aART-2020-116402
000095868 041__ $$aeng
000095868 100__ $$aNonno, R
000095868 245__ $$aCharacterization of goat prions demonstrates geographical variation of scrapie strains in Europe and reveals the composite nature of prion strains
000095868 260__ $$c2020
000095868 5060_ $$aAccess copy available to the general public$$fUnrestricted
000095868 5203_ $$aBovine Spongiform Encephalopathy (BSE) is the only animal prion which has been recognized as a zoonotic agent so far. The identification of BSE in two goats raised the need to reliably identify BSE in small ruminants. However, our understanding of scrapie strain diversity in small ruminants remains ill-defined, thus limiting the accuracy of BSE surveillance and spreading fear that BSE might lurk unrecognized in goats. We investigated prion strain diversity in a large panel of European goats by a novel experimental approach that, instead of assessing the neuropathological profile after serial transmissions in a single animal model, was based on the direct interaction of prion isolates with several recipient rodent models expressing small ruminants or heterologous prion proteins. The findings show that the biological properties of scrapie isolates display different patterns of geographical distribution in Europe and suggest that goat BSE could be reliably discriminated from a wide range of biologically and geographically diverse goat prion isolates. Finally, most field prion isolates showed composite strain features, with discrete strain components or sub-strains being present in different proportions in individual goats or tissues. This has important implications for understanding the nature and evolution of scrapie strains and their transmissibility to other species, including humans.
000095868 536__ $$9info:eu-repo/grantAgreement/EC/FP6/36353/EU/Goat BSE: proposal for improvement of goat TSE discriminative diagnosis and susceptibility based assessment of BSE infectivity in goat milk and meat/GOATBSE$$9info:eu-repo/grantAgreement/ES/INIA/FPI-SGIT-2015-02$$9info:eu-repo/grantAgreement/ES/MCIU-AEI-FEDER/AGL2016-78054-R$$9info:eu-repo/grantAgreement/ES/MCIU/AGL2012-37988-C04-04$$9info:eu-repo/grantAgreement/ES/MINECO/BES-2010-040922
000095868 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000095868 590__ $$a4.379$$b2020
000095868 591__ $$aMULTIDISCIPLINARY SCIENCES$$b17 / 73 = 0.233$$c2020$$dQ1$$eT1
000095868 592__ $$a1.24$$b2020
000095868 593__ $$aMultidisciplinary$$c2020$$dQ1
000095868 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000095868 700__ $$aMarin-Moreno, A
000095868 700__ $$aEspinosa, JC
000095868 700__ $$aFast, C
000095868 700__ $$aVan Keulen, L
000095868 700__ $$aSpiropoulos, J
000095868 700__ $$aLantier, I
000095868 700__ $$aAndreoletti, O
000095868 700__ $$aPirisinu, L
000095868 700__ $$aDi Bari, MA
000095868 700__ $$aAguilar-Calvo, P
000095868 700__ $$aSklaviadis, T
000095868 700__ $$aPapasavva-Stylianou, P
000095868 700__ $$aAcutis, PL
000095868 700__ $$0(orcid)0000-0001-5105-6133$$aAcin, C$$uUniversidad de Zaragoza
000095868 700__ $$aBossers, A
000095868 700__ $$aJacobs, JG
000095868 700__ $$aVaccari, G
000095868 700__ $$aD''Agostino, C
000095868 700__ $$aChiappini, B
000095868 700__ $$aLantier, F
000095868 700__ $$aGroschup, MH
000095868 700__ $$aAgrimi, U
000095868 700__ $$aTorres, JM
000095868 700__ $$aLangeveld, JPM
000095868 7102_ $$11009$$2773$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Sanidad Animal
000095868 773__ $$g10 (2020), 19 [15 pp]$$pSci. rep.$$tScientific Reports$$x2045-2322
000095868 8564_ $$s2255268$$uhttps://zaguan.unizar.es/record/95868/files/texto_completo.pdf$$yVersión publicada
000095868 8564_ $$s215189$$uhttps://zaguan.unizar.es/record/95868/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000095868 909CO $$ooai:zaguan.unizar.es:95868$$particulos$$pdriver
000095868 951__ $$a2021-09-02-08:46:58
000095868 980__ $$aARTICLE