000095879 001__ 95879 000095879 005__ 20210902121642.0 000095879 0247_ $$2doi$$a10.3390/ani10020261 000095879 0248_ $$2sideral$$a116790 000095879 037__ $$aART-2020-116790 000095879 041__ $$aeng 000095879 100__ $$0(orcid)0000-0001-8932-4615$$aAmanzougarene, Z. 000095879 245__ $$aFermentation pattern of several carbohydrate sources incubated in an in vitro semicontinuous system with inocula from ruminants given either forage or concentrate-based diets 000095879 260__ $$c2020 000095879 5060_ $$aAccess copy available to the general public$$fUnrestricted 000095879 5203_ $$aThe fermentation pattern of several carbohydrate sources and their interaction with the nature of microbial inoculum was studied. Barley (B), maize (M), sorghum, (S), sugarbeet pulp (BP), citrus pulp (CP) and wheat bran (WB) were tested in an in vitro semicontinuous system maintaining poorly buffered conditions from 0 to 6 h, and being gradually buffered to 6.5 from 8 to 24 h to simulate the rumen pH pattern. Rumen fluid inoculum was obtained from lambs fed with either concentrate and barley straw (CI) or alfalfa hay (FI). The extent of fermentation was higher with CI than FI throughout the incubation (p < 0.05). Among the substrates, S, BP and M maintained the highest pH (p < 0.05), whereas CP recorded the lowest pH with both inocula. Similarly, CP recorded the highest gas volume throughout the incubation, followed by WB and B, and S recorded the lowest volume (p < 0.05). On average, the total volatile fatty acid (VFA), as well as lactic acid concentration, was higher with CP than in the other substrates (p < 0.05). The microbial structure was more affected by the animal donor of inoculum than by the substrate. The in vitro semicontinuous system allows for the study of the rumen environment acidification and substrate microbial fermentation under intensive feeding conditions. © 2020 by the authors. Licensee MDPI, Basel, Switzerland. 000095879 536__ $$9info:eu-repo/grantAgreement/ES/MECD/FPU-Formación de profesorado universitario$$9info:eu-repo/grantAgreement/ES/MINECO-DGA-FSE/AGL2013-46820 000095879 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/ 000095879 590__ $$a2.752$$b2020 000095879 591__ $$aVETERINARY SCIENCES$$b19 / 146 = 0.13$$c2020$$dQ1$$eT1 000095879 591__ $$aAGRICULTURE, DAIRY & ANIMAL SCIENCE$$b13 / 63 = 0.206$$c2020$$dQ1$$eT1 000095879 592__ $$a0.583$$b2020 000095879 593__ $$aVeterinary (miscellaneous)$$c2020$$dQ1 000095879 593__ $$aAnimal Science and Zoology$$c2020$$dQ1 000095879 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion 000095879 700__ $$0(orcid)0000-0003-3927-9793$$aYuste, S. 000095879 700__ $$0(orcid)0000-0002-0712-1185$$aFondevila, M.$$uUniversidad de Zaragoza 000095879 7102_ $$12008$$2700$$aUniversidad de Zaragoza$$bDpto. Produc.Animal Cienc.Ali.$$cÁrea Producción Animal 000095879 773__ $$g10, 2 (2020), 261 [14 pp]$$pAnimals (Basel)$$tAnimals$$x2076-2615 000095879 8564_ $$s488289$$uhttps://zaguan.unizar.es/record/95879/files/texto_completo.pdf$$yVersión publicada 000095879 8564_ $$s479419$$uhttps://zaguan.unizar.es/record/95879/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada 000095879 909CO $$ooai:zaguan.unizar.es:95879$$particulos$$pdriver 000095879 951__ $$a2021-09-02-09:00:40 000095879 980__ $$aARTICLE