95879 20210902121642.0 doi 10.3390/ani10020261 sideral 116790 ART-2020-116790 eng Amanzougarene, Z. (orcid)0000-0001-8932-4615 Fermentation pattern of several carbohydrate sources incubated in an in vitro semicontinuous system with inocula from ruminants given either forage or concentrate-based diets 2020 Access copy available to the general public Unrestricted The fermentation pattern of several carbohydrate sources and their interaction with the nature of microbial inoculum was studied. Barley (B), maize (M), sorghum, (S), sugarbeet pulp (BP), citrus pulp (CP) and wheat bran (WB) were tested in an in vitro semicontinuous system maintaining poorly buffered conditions from 0 to 6 h, and being gradually buffered to 6.5 from 8 to 24 h to simulate the rumen pH pattern. Rumen fluid inoculum was obtained from lambs fed with either concentrate and barley straw (CI) or alfalfa hay (FI). The extent of fermentation was higher with CI than FI throughout the incubation (p < 0.05). Among the substrates, S, BP and M maintained the highest pH (p < 0.05), whereas CP recorded the lowest pH with both inocula. Similarly, CP recorded the highest gas volume throughout the incubation, followed by WB and B, and S recorded the lowest volume (p < 0.05). On average, the total volatile fatty acid (VFA), as well as lactic acid concentration, was higher with CP than in the other substrates (p < 0.05). The microbial structure was more affected by the animal donor of inoculum than by the substrate. The in vitro semicontinuous system allows for the study of the rumen environment acidification and substrate microbial fermentation under intensive feeding conditions. © 2020 by the authors. Licensee MDPI, Basel, Switzerland. info:eu-repo/grantAgreement/ES/MECD/FPU-Formación de profesorado universitario info:eu-repo/grantAgreement/ES/MINECO-DGA-FSE/AGL2013-46820 info:eu-repo/semantics/openAccess by http://creativecommons.org/licenses/by/3.0/es/ 2.752 2020 VETERINARY SCIENCES 19 / 146 = 0.13 2020 Q1 T1 AGRICULTURE, DAIRY & ANIMAL SCIENCE 13 / 63 = 0.206 2020 Q1 T1 0.583 2020 Veterinary (miscellaneous) 2020 Q1 Animal Science and Zoology 2020 Q1 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Yuste, S. (orcid)0000-0003-3927-9793 Fondevila, M. Universidad de Zaragoza (orcid)0000-0002-0712-1185 2008 700 Universidad de Zaragoza Dpto. Produc.Animal Cienc.Ali. Área Producción Animal 10, 2 (2020), 261 [14 pp] Animals (Basel) Animals 2076-2615 488289 http://zaguan.unizar.es/record/95879/files/texto_completo.pdf Versión publicada 479419 http://zaguan.unizar.es/record/95879/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:95879 articulos driver 2021-09-02-09:00:40 ARTICLE