000095903 001__ 95903
000095903 005__ 20210902121650.0
000095903 0247_ $$2doi$$a10.3389/fpls.2019.01780
000095903 0248_ $$2sideral$$a116847
000095903 037__ $$aART-2020-116847
000095903 041__ $$aeng
000095903 100__ $$aEscudero, V.
000095903 245__ $$aNicotianamine Synthase 2 Is Required for Symbiotic Nitrogen Fixation in Medicago truncatula Nodules
000095903 260__ $$c2020
000095903 5060_ $$aAccess copy available to the general public$$fUnrestricted
000095903 5203_ $$aSymbiotic nitrogen fixation carried out by the interaction between legumes and diazotrophic bacteria known as rhizobia requires relatively large levels of transition metals. These elements are cofactors of many key enzymes involved in this process. Metallic micronutrients are obtained from soil by the roots and directed to sink organs by the vasculature, in a process mediated by a number of metal transporters and small organic molecules that facilitate metal delivery in the plant fluids. Among the later, nicotianamine is one of the most important. Synthesized by nicotianamine synthases (NAS), this molecule forms metal complexes participating in intracellular metal homeostasis and long-distance metal trafficking. Here we characterized the NAS2 gene from model legume Medicago truncatula. MtNAS2 is located in the root vasculature and in all nodule tissues in the infection and fixation zones. Symbiotic nitrogen fixation requires of MtNAS2 function, as indicated by the loss of nitrogenase activity in the insertional mutant nas2-1, phenotype reverted by reintroduction of a wild-type copy of MtNAS2. This would result from the altered iron distribution in nas2-1 nodules shown with X-ray fluorescence. Moreover, iron speciation is also affected in these nodules. These data suggest a role of nicotianamine in iron delivery for symbiotic nitrogen fixation.
000095903 536__ $$9info:eu-repo/grantAgreement/EC/FP7/335284/EU/Metal transport in the tripartite symbiosis arbuscular mycorrhizal fungi-legume-rhizobia/METALSYM$$9info:eu-repo/grantAgreement/ES/MINECO/AGL2015-65866-P$$9info:eu-repo/grantAgreement/ES/MINECO/AGL2016-75226-R
000095903 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000095903 590__ $$a5.753$$b2020
000095903 591__ $$aPLANT SCIENCES$$b17 / 235 = 0.072$$c2020$$dQ1$$eT1
000095903 592__ $$a1.752$$b2020
000095903 593__ $$aPlant Science$$c2020$$dQ1
000095903 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000095903 700__ $$aAbreu, I.
000095903 700__ $$adel Sastre, E.
000095903 700__ $$aTejada-Jiménez, M.
000095903 700__ $$aLarue, C.
000095903 700__ $$aNovoa-Aponte, L.
000095903 700__ $$aCastillo-González, J.
000095903 700__ $$aWen, J.
000095903 700__ $$aMysore, K. S.
000095903 700__ $$0(orcid)0000-0001-5470-5901$$aAbadía, J.
000095903 700__ $$aArgüello, J. M.
000095903 700__ $$aCastillo-Michel, H.
000095903 700__ $$0(orcid)0000-0003-4568-1201$$aÁlvarez-Fernández, A.$$uUniversidad de Zaragoza
000095903 700__ $$aImperial, J.
000095903 700__ $$aGonzález-Guerrero, M.
000095903 7102_ $$15011$$2705$$aUniversidad de Zaragoza$$bDpto. CC.Agrar.y Medio Natural$$cÁrea Producción Vegetal
000095903 773__ $$g10 (2020), 1780 [14 pp]$$pFront. plant sci.$$tFrontiers in Plant Science$$x1664-462X
000095903 8564_ $$s722604$$uhttps://zaguan.unizar.es/record/95903/files/texto_completo.pdf$$yVersión publicada
000095903 8564_ $$s25239$$uhttps://zaguan.unizar.es/record/95903/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000095903 909CO $$ooai:zaguan.unizar.es:95903$$particulos$$pdriver
000095903 951__ $$a2021-09-02-09:05:54
000095903 980__ $$aARTICLE